Anti-microbial hydroxyapatite particles synthesized by a sol-gel route

Sol–gel route was applied to synthesize anti-microbial hydroxyapatite (HAp) powders by the addition of silver 200–20000 ppm or zinc salts. The bacteria strain, Streptococcus mutans (S. mutans, ATCC 25175) was used in the anti-bacterial tests. HAp phase was reproducibly obtained by the preparation co...

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Bibliographic Details
Published in:Journal of sol-gel science and technology 2005-02, Vol.33 (2), p.229-239
Main Authors: CHUNG, Ren-Jei, HSIEH, Ming-Fa, HUANG, Kuo-Chang, PERNG, Li-Hsiang, CHOU, Fong-In, CHIN, Tsung-Shune
Format: Article
Language:English
Subjects:
Antiinfectives and antibacterials
Atomic emission spectroscopy
Brain
Calcium
Chemistry
Colloidal gels. Colloidal sols
Colloidal state and disperse state
Emission analysis
Ethanol
Exact sciences and technology
Gelation
General and physical chemistry
Hydroxyapatite
Inductively coupled plasma
Microorganisms
Organic chemistry
Roasting
Silver
Sol-gel processes
Solid state
Streptococcus mutans
Synthesis
Thermodynamic properties
Zinc salts
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Summary:Sol–gel route was applied to synthesize anti-microbial hydroxyapatite (HAp) powders by the addition of silver 200–20000 ppm or zinc salts. The bacteria strain, Streptococcus mutans (S. mutans, ATCC 25175) was used in the anti-bacterial tests. HAp phase was reproducibly obtained by the preparation conditions: Ca(NO3)2-4H2O and trietheyl phosphate as sources of calcium and phosphorus sources, respectively, and ethanol as the solvent, aging for 16 h at 80°C, gelation and drying at 80°C for 24 h, then calcining at above 350°C. TGA was used to analyze thermal properties of the as-prepared gel. XRD and FTIR were used to identify the crystalline phase and chemical structure. CaO appeared as an impurity after calcining above 650°C. In the solid-state anti-microbial tests on the brain heart infusion (BHI) agar plates, there formed a microbial inhibition zone surrounding the Ag/Zn added (greater than 2000 ppm) samples. In the liquid-state anti-microbial tests, S. mutans cells readily precipitated with pure HAp powders but not with Ag/Zn added (greater than 2000 ppm) HAp powders. The concentration of silver or zinc ions releasing from the Ag/Zn added HAp powders into the supernatant of the BHI broth was under detection limit of ICP-AES analyses. However, the growth of S. mutans reached same magnitude (6 × 108 CFU/mL) whether pure HAp, 2000 ppm-Ag or 2000 ppm-Zn were added. Therefore, Ag/Zn added HAp powders developed in this research present microbial inhibition properties and are of potential as a solid-state anti-microbial agent.
ISSN:0928-0707
1573-4846
DOI:10.1007/s10971-005-5618-1