Antioxidant properties of water-soluble polysaccharides from Antrodia cinnamomea in submerged culture

Antrodia cinnamomea, a well-known tradition Chinese medicine, possesses anti-tumor, anti-oxidation activities and stimulates the immune system. The aim of this study was to investigate the protective effect of water-soluble polysaccharides from the fermented filtrate and mycelia of Antrodia cinnamom...

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Bibliographic Details
Published in:Food chemistry 2007, Vol.104 (3), p.1115-1122
Main Authors: Tsai, Ming-Chi, Song, Tuzz-Ying, Shih, Ping-Hsiao, Yen, Gow-Chin
Format: Article
Language:English
Subjects:
Antioxidant
antioxidant activity
Antrodia cinnamomea
Basidiomycota
Biological and medical sciences
cell culture
Chang liver cells
Cinnamonum kanehirai
cytotoxicity
DNA damage
Food industries
Fundamental and applied biological sciences. Psychology
fungi
hepatocytes
lipid peroxidation
medicinal plants
medicinal properties
mycelium
Oriental traditional medicine
oxidative stress
parasitoses
Polysaccharide
polysaccharides
protective effect
protein content
solubility
submerged fermentation
sugar content
thiobarbituric acid-reactive substances
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Summary:Antrodia cinnamomea, a well-known tradition Chinese medicine, possesses anti-tumor, anti-oxidation activities and stimulates the immune system. The aim of this study was to investigate the protective effect of water-soluble polysaccharides from the fermented filtrate and mycelia of Antrodia cinnamomea in submerged culture (ACSC) on hydrogen peroxide-induced cytotoxicity and DNA damage in Chang liver cells. Oxidative DNA damage was evaluated by single cell gel electrophoresis (Comet assay) or by the formation of 8-hydroxy-deoxyguanosine (8-OHdG) adducts. The polysaccharides isolated by ion-exchange chromatography contained glucose, xylose, galactose, arabinose, and mannose. The results showed that incubation of Chang liver cells with isolated polysaccharides at 200 μg/mL for 5 h prior to H 2O 2 treatment (50 μM, 30 min) significantly reduced oxidative DNA damage as detected by the formation of comet tail DNA and 8-OHdG adducts by 89% and 69%, respectively. Pre-treatment Chang liver cells with polysaccharides also reduced the levels of thiobarbituric acid reactive substances (TBARS) ( p < 0.01) and intracellular reactive species (ROS) ( p < 0.01) induced by H 2O 2. Moreover, glutathione S-transferase (GST) and the GSH/GSSG ratio were significantly increased in Chang liver cells pre-incubated with the polysaccharides ( p < 0.01). These results demonstrate that polysaccharides in ASCS have antioxidant properties which may involve up-regulation of GST activity, maintenance of normal GSH/GSSG ratio, and scavenging of ROS.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2007.01.018