First certified reference materials for molecular fingerprinting of two approved probiotic Bacillus strains

At present probiotic bacteria are widely used in human and animal nutrition because they beneficially influence the balance of the intestinal flora of the host. Positive effects related to probiotics are various and include enhancement of digestion, strengthening of the immune system and stimulation...

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Bibliographic Details
Published in:International journal of food microbiology 2009-01, Vol.129 (1), p.16-20
Main Authors: De Baets, L., Van Iwaarden, P., Meeus, N., Schimmel, H., Philipp, W., Emons, H.
Format: Article
Language:English
Subjects:
Animals
Bacillus
Bacillus - genetics
Bacillus licheniformis
Bacillus subtilis
Base Sequence
Biological and medical sciences
Colony Count, Microbial
CRM
DNA conformation
DNA Fingerprinting
DNA Primers
DNA, Bacterial - analysis
Electrophoresis, Gel, Pulsed-Field
European Union
Feed and pet food industries
Feed law
feed laws
feeds
Food Analysis - standards
Food industries
Food Labeling - standards
Food Microbiology
Fundamental and applied biological sciences. Psychology
genomics
Humans
lactic acid bacteria
laws and regulations
molecular systematics
PFGE
phenotype
Probiotics
pulsed-field gel electrophoresis
Reference Standards
RNA, Ribosomal, 16S - genetics
RNA, Ribosomal, 23S - genetics
Sequence Alignment
Species Specificity
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Summary:At present probiotic bacteria are widely used in human and animal nutrition because they beneficially influence the balance of the intestinal flora of the host. Positive effects related to probiotics are various and include enhancement of digestion, strengthening of the immune system and stimulation of vitamin production. Moreover, implementation of probiotics is intended to reduce the use of antibiotics and improve animal growth and feed conversion. To protect human and animal health and to improve consumer confidence, a strict legislation on the use of probiotics exists within the European Union (EU). Official controls by national authorities are performed to ensure verification of compliance with feed and food law. Apart from the risk of using unauthorized strains, mislabelling is a known problem, partly because of the use of phenotyping or genotyping methods with a lack of discriminative power. In addition to official controls, private controls by food and feed producing companies are important in the frame of protection of patented strains and industrial property rights. To support these applications, IRMM has developed certified reference materials (CRMs) consisting of genomic DNA inserts of B. subtilis DSM 5749 and B. licheniformis DSM 5750, two strains that received EU approval. In this study we investigated the use of these CRMs, IRMM-311 and IRMM-312, for the detection and unambiguous discrimination of Bacillus strains by pulsed-field gel electrophoresis (PFGE). Identical fingerprints were obtained for the CRMs and control strains isolated from the feed additive Bioplus 2B ®. On the other hand a distinction could be made from other not approved B. licheniformis and B. subtilis strains. The reference materials discussed in this study are the first CRMs based on a whole bacterial genome and suitable for PFGE. They offer perspectives for applications in other domains such as analysis of foodborne pathogens in outbreaks or routine analysis.
ISSN:0168-1605
1879-3460
DOI:10.1016/j.ijfoodmicro.2008.10.028