Characterization of gene expression profiles in developing kernels of maize (Zea mays) inbred Tex6

Maize inbred Tex6 is resistant to several pests. The objectives of this study were to characterize gene expression profiles in Tex6 kernels and identify unique genes that expressed up- or down-ward in developing kernels in the later stages. Because of the resistance of Tex6 kernels, we reasoned that...

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Bibliographic Details
Published in:Plant breeding 2008-12, Vol.127 (6), p.569-578
Main Authors: Luo, M, Liu, J, Lee, R.D, Guo, B.Z
Format: Article
Language:English
Subjects:
aflatoxin
Agronomy. Soil science and plant productions
Aspergillus flavus
Biological and medical sciences
carbohydrate metabolism
developing kernels
disease resistance
Fundamental and applied biological sciences. Psychology
gene expression
gene expression regulation
Genetics and breeding of economic plants
lipid metabolism
microarray technology
resistance genes
reverse transcriptase polymerase chain reaction
seed development
seeds
starch
storage proteins
stress tolerance
Varietal selection. Specialized plant breeding, plant breeding aims
Zea mays
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Summary:Maize inbred Tex6 is resistant to several pests. The objectives of this study were to characterize gene expression profiles in Tex6 kernels and identify unique genes that expressed up- or down-ward in developing kernels in the later stages. Because of the resistance of Tex6 kernels, we reasoned that it would be an interesting candidate for microarray study. By using maize microarray, we analysed gene expression profiles in developing kernels from 25 DAP (days after pollination) to 45 DAP. A total of 8497 positive array spots were detected with unique IDs, in which 4247 genes were detected in all samples. The trends of total expressed genes were decreasing when kernels were maturing. Expression patterns of some genes in several metabolic pathways, including starch, lipid and storage proteins, were analysed. In comparison with 25 DAP, expressions of 211 gene features were significantly different at 45 DAP (P < 0.05), which will be used to produce a macroarray for germplasm assessment and evaluation. The real-time qRT-PCR was used to validate microarray study.
ISSN:0179-9541
1439-0523
DOI:10.1111/j.1439-0523.2008.01538.x