Isolation and characterization of two hydrocarbon-degrading Bacillus subtilis strains from oil contaminated soil of Kuwait

Two strains of hydrocarbon-utilizing bacteria were isolated from soil samples of the Kuwait Burqan oil field at a temperature of 37°C. The bacteria were motile endospore-forming rods with slight differences in their metabolic patterns and 16S rRNA sequence. Vegetative cells of the strains designated...

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Bibliographic Details
Published in:Microbiological research 2000-09, Vol.155 (3), p.157-164
Main Authors: Al-Sharidah, Ahlam, Richardt, Andre, Golecki, Jochen R., Dierstein, Roland, Tadros, Monier H.
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Alkanes - metabolism
Bacillus subtilis
Bacillus subtilis - isolation & purification
Bacillus subtilis - metabolism
Bacillus subtilis - ultrastructure
Bacterial Typing Techniques
Biodegradation of pollutants
Biological and medical sciences
Biotechnology
Environment and pollution
Fundamental and applied biological sciences. Psychology
Gasoline
hydrocarbon biodegradation
Hydrocarbons - metabolism
Industrial applications and implications. Economical aspects
Kuwait
morphology
Naphthalenes - metabolism
Petroleum - metabolism
physiology
RNA, Ribosomal, 16S - genetics
Soil and water pollution
Soil Microbiology
Soil Pollutants - metabolism
Soil science
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Summary:Two strains of hydrocarbon-utilizing bacteria were isolated from soil samples of the Kuwait Burqan oil field at a temperature of 37°C. The bacteria were motile endospore-forming rods with slight differences in their metabolic patterns and 16S rRNA sequence. Vegetative cells of the strains designated as AHI and AHII had an ultrastructure typical of Gram-positive bacteria and showed Gram-positive staining. The bacteria did not show pigmentation. Best growth was observed at 37°C at neutral pH and NaCl concentrations in the range of 5–10 g per 1. Both strains were obligatory aerobic and developed on synthetic media with either Diesel fuel, n-decan or naphthalene as the sole carbon and energy source. No specific growth factors were required. On the basis of their morphological, physiological and biochemical features, as well as their 16S rRNA analysis and electron microscope study, both strains were assigned to the species of Bacillus subtilis.
ISSN:0944-5013
1618-0623
DOI:10.1016/S0944-5013(00)80029-4