Reductive tetrachloroethene dehalogenation in the presence of oxygen by Sulfurospirillum multivorans: physiological studies and proteome analysis

Abstract Reductive dehalogenation of organohalides is carried out by organohalide-respiring bacteria (OHRB) in anoxic environments. The tetrachloroethene (PCE)-respiring Epsilonproteobacterium Sulfurospirillum multivorans is one of few OHRB able to respire oxygen. Therefore, we investigated the orga...

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Published in:FEMS microbiology ecology 2018-01, Vol.94 (1), p.1
Main Authors: Gadkari, Jennifer, Goris, Tobias, Schiffmann, Christian L, Rubick, Raffael, Adrian, Lorenz, Schubert, Torsten, Diekert, Gabriele
Format: Article
Language:English
Subjects:
Anoxic conditions
Bacteria
Bioremediation
Catalase
Chemical properties
Dechlorination
Dehalogenation
Ecology
Environmental aspects
Gram-negative bacteria
Hydrogen peroxide
Methods
Microbiology
Oxidation
Oxygen
Perchloroethylene
Peroxidase
Peroxide reductase
Physiological aspects
Proteomes
Redox potential
Superoxide
Tetrachloroethylene
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Summary:Abstract Reductive dehalogenation of organohalides is carried out by organohalide-respiring bacteria (OHRB) in anoxic environments. The tetrachloroethene (PCE)-respiring Epsilonproteobacterium Sulfurospirillum multivorans is one of few OHRB able to respire oxygen. Therefore, we investigated the organism's capacity to dehalogenate PCE in the presence of oxygen, which would broaden the applicability to use S. multivorans, unlike other commonly oxygen-sensitive OHRB, for bioremediation, e.g. at oxic/anoxic interphases. Additionally, this has an impact on our understanding of the global halogen cycle. Sulfurospirillum multivorans performs dehalogenation of PCE to cis-1,2-dichloroethene at oxygen concentrations below 0.19 mg/L. The redox potential of the medium electrochemically adjusted up to +400 mV had no influence on reductive dehalogenation by S. multivorans in our experiments, suggesting that higher levels of oxygen impair PCE dechlorination by inhibiting or inactivating involved enzymes. The PCE reductive dehalogenase remained active in cell extracts of S. multivorans exposed to 0.37 mg/L oxygen for more than 96 h. Analysis of the proteome revealed that superoxide reductase and cytochrome peroxidase amounts increased with 5% oxygen in the gas phase, while the response to atmospheric oxygen concentrations involved catalase and hydrogen peroxide reductase. Taken together, our results demonstrate that reductive dehalogenation by OHRB is not limited to anoxic conditions. Opposed to other organohalide-respiring bacteria, Sulfurospirillum multivorans is able to reductively dechlorinate tetrachloroethene to cis-dichloroethene under low oxygen concentrations
ISSN:1574-6941
0168-6496
1574-6941
DOI:10.1093/femsec/fix176