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In vitro evaluation of methylene blue removal from root canal after Photodynamic Therapy
•The importance of cleaning the dentin after Photodynamic Therapy.•Test saline, NaOCl, EDTA and PUI to remove methylene blue of root canal.•No treatments completely remove methylene blue from the root canal after PDT. In Endodontics, photosensitizers’ such as methylene blue and toluidine blue have b...
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| Published in: | Photodiagnosis and photodynamic therapy 2017-12, Vol.20, p.248-252 |
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| container_title | Photodiagnosis and photodynamic therapy |
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| creator | Ramalho, Karen Müller Cunha, Sandra Ribeiro Mayer-Santos, Eric Eduardo, Carlos de Paula Freitas, Patricia Moreira de Aranha, Ana Cecilia Correa Moura-Netto, Cacio |
| description | •The importance of cleaning the dentin after Photodynamic Therapy.•Test saline, NaOCl, EDTA and PUI to remove methylene blue of root canal.•No treatments completely remove methylene blue from the root canal after PDT.
In Endodontics, photosensitizers’ such as methylene blue and toluidine blue have been used in Photodynamic Therapy due to their positive results. However, they can stain the dentin from the root canal after Photodynamic Therapy (PDT). The present in vitro study aimed to evaluate different stain removal protocols from root canal after PDT using methylene blue (MB) dye. After mechanical preparation of the root canal of 40 uni-radicular human teeth, PDT was performed using 0,01% MB with parameters of 3 min of pre-irradiation and a diode laser irradiation emitting at 660 nm, 40 mW, 4 min, 9.6 J. After PDT, different protocols of MB removal were performed: Group 1 − control (0.9% saline solution); Group 2 − sodium hypochlorite (2.5% NaOCl); Group 3–17% ethylenediamine tetraacetic acid (EDTA); Group 4 − passive ultrasonic irrigation (PUI); The color of the dentin of the root canal was measured, before, immediately after the PDT and immediately after the cleaning using a spectrophotometer. The ΔE values found were statistically compared using the ANOVA and Tukey's tests (α=0.05). All the treatments lead to some cleaning of root canal after PDT, however, none of the treatments tested completely removed all staining caused by MB photosensitizer of the root canal. Among the treatments tested, PUI and Hypochlorite 2.5% promoted greater cleaning, with no statistically significant difference between them. In conclusion, within the protocols tested in the present study, no treatments were able to completely remove MB staining of the root canal after PDT. |
| doi_str_mv | 10.1016/j.pdpdt.2017.10.024 |
| format | article |
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In Endodontics, photosensitizers’ such as methylene blue and toluidine blue have been used in Photodynamic Therapy due to their positive results. However, they can stain the dentin from the root canal after Photodynamic Therapy (PDT). The present in vitro study aimed to evaluate different stain removal protocols from root canal after PDT using methylene blue (MB) dye. After mechanical preparation of the root canal of 40 uni-radicular human teeth, PDT was performed using 0,01% MB with parameters of 3 min of pre-irradiation and a diode laser irradiation emitting at 660 nm, 40 mW, 4 min, 9.6 J. After PDT, different protocols of MB removal were performed: Group 1 − control (0.9% saline solution); Group 2 − sodium hypochlorite (2.5% NaOCl); Group 3–17% ethylenediamine tetraacetic acid (EDTA); Group 4 − passive ultrasonic irrigation (PUI); The color of the dentin of the root canal was measured, before, immediately after the PDT and immediately after the cleaning using a spectrophotometer. The ΔE values found were statistically compared using the ANOVA and Tukey's tests (α=0.05). All the treatments lead to some cleaning of root canal after PDT, however, none of the treatments tested completely removed all staining caused by MB photosensitizer of the root canal. Among the treatments tested, PUI and Hypochlorite 2.5% promoted greater cleaning, with no statistically significant difference between them. In conclusion, within the protocols tested in the present study, no treatments were able to completely remove MB staining of the root canal after PDT.</description><identifier>ISSN: 1572-1000</identifier><identifier>EISSN: 1873-1597</identifier><identifier>DOI: 10.1016/j.pdpdt.2017.10.024</identifier><identifier>PMID: 29097290</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Dental Pulp Cavity - drug effects ; Edetic Acid - therapeutic use ; Endodontics ; Humans ; Lasers, Semiconductor ; Methylene blue ; Methylene Blue - adverse effects ; Methylene Blue - therapeutic use ; Photochemotherapy - methods ; Photodynamic Therapy ; Photosensitizing Agents - adverse effects ; Photosensitizing Agents - therapeutic use ; Random Allocation ; Root Canal Irrigants - therapeutic use ; Sodium Hypochlorite - therapeutic use ; Tolonium Chloride - therapeutic use</subject><ispartof>Photodiagnosis and photodynamic therapy, 2017-12, Vol.20, p.248-252</ispartof><rights>2017 Elsevier B.V.</rights><rights>Copyright © 2017 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-72d1abe980cd632537c3c9a7ad5f4535db937ebe6b00d50db540525174b92ec83</citedby><cites>FETCH-LOGICAL-c359t-72d1abe980cd632537c3c9a7ad5f4535db937ebe6b00d50db540525174b92ec83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29097290$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramalho, Karen Müller</creatorcontrib><creatorcontrib>Cunha, Sandra Ribeiro</creatorcontrib><creatorcontrib>Mayer-Santos, Eric</creatorcontrib><creatorcontrib>Eduardo, Carlos de Paula</creatorcontrib><creatorcontrib>Freitas, Patricia Moreira de</creatorcontrib><creatorcontrib>Aranha, Ana Cecilia Correa</creatorcontrib><creatorcontrib>Moura-Netto, Cacio</creatorcontrib><title>In vitro evaluation of methylene blue removal from root canal after Photodynamic Therapy</title><title>Photodiagnosis and photodynamic therapy</title><addtitle>Photodiagnosis Photodyn Ther</addtitle><description>•The importance of cleaning the dentin after Photodynamic Therapy.•Test saline, NaOCl, EDTA and PUI to remove methylene blue of root canal.•No treatments completely remove methylene blue from the root canal after PDT.
In Endodontics, photosensitizers’ such as methylene blue and toluidine blue have been used in Photodynamic Therapy due to their positive results. However, they can stain the dentin from the root canal after Photodynamic Therapy (PDT). The present in vitro study aimed to evaluate different stain removal protocols from root canal after PDT using methylene blue (MB) dye. After mechanical preparation of the root canal of 40 uni-radicular human teeth, PDT was performed using 0,01% MB with parameters of 3 min of pre-irradiation and a diode laser irradiation emitting at 660 nm, 40 mW, 4 min, 9.6 J. After PDT, different protocols of MB removal were performed: Group 1 − control (0.9% saline solution); Group 2 − sodium hypochlorite (2.5% NaOCl); Group 3–17% ethylenediamine tetraacetic acid (EDTA); Group 4 − passive ultrasonic irrigation (PUI); The color of the dentin of the root canal was measured, before, immediately after the PDT and immediately after the cleaning using a spectrophotometer. The ΔE values found were statistically compared using the ANOVA and Tukey's tests (α=0.05). All the treatments lead to some cleaning of root canal after PDT, however, none of the treatments tested completely removed all staining caused by MB photosensitizer of the root canal. Among the treatments tested, PUI and Hypochlorite 2.5% promoted greater cleaning, with no statistically significant difference between them. In conclusion, within the protocols tested in the present study, no treatments were able to completely remove MB staining of the root canal after PDT.</description><subject>Dental Pulp Cavity - drug effects</subject><subject>Edetic Acid - therapeutic use</subject><subject>Endodontics</subject><subject>Humans</subject><subject>Lasers, Semiconductor</subject><subject>Methylene blue</subject><subject>Methylene Blue - adverse effects</subject><subject>Methylene Blue - therapeutic use</subject><subject>Photochemotherapy - methods</subject><subject>Photodynamic Therapy</subject><subject>Photosensitizing Agents - adverse effects</subject><subject>Photosensitizing Agents - therapeutic use</subject><subject>Random Allocation</subject><subject>Root Canal Irrigants - therapeutic use</subject><subject>Sodium Hypochlorite - therapeutic use</subject><subject>Tolonium Chloride - therapeutic use</subject><issn>1572-1000</issn><issn>1873-1597</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp9kE1v1DAQhi0EoqXlFyAhH7lkGdvxOj5wQFWBSpXooUi9WY490XqVxMF2Vtp_Xy9bOHKZz3dmNA8hHxhsGLDt5_1m8YsvGw5M1coGePuKXLJOiYZJrV7XWCreMAC4IO9y3gOIVkP7llxwDVpVc0me7mZ6CCVFigc7rraEONM40AnL7jjijLQfV6QJp1j7dEhxoinGQp2da26Hgok-7GKJ_jjbKTj6uMNkl-M1eTPYMeP7F39Ffn27fbz50dz__H538_W-cULq0ijume1Rd-D8VnAplBNOW2W9HFoppO-1UNjjtgfwEnwvW5BcMtX2mqPrxBX5dN67pPh7xVzMFLLDcbQzxjUbppWoi7qOV6k4S12KOScczJLCZNPRMDAnpGZv_iA1J6SnYkVapz6-HFj7Cf2_mb8Mq-DLWYD1zUPAZLILODv0IaErxsfw3wPPv6yJSw</recordid><startdate>201712</startdate><enddate>201712</enddate><creator>Ramalho, Karen Müller</creator><creator>Cunha, Sandra Ribeiro</creator><creator>Mayer-Santos, Eric</creator><creator>Eduardo, Carlos de Paula</creator><creator>Freitas, Patricia Moreira de</creator><creator>Aranha, Ana Cecilia Correa</creator><creator>Moura-Netto, Cacio</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201712</creationdate><title>In vitro evaluation of methylene blue removal from root canal after Photodynamic Therapy</title><author>Ramalho, Karen Müller ; Cunha, Sandra Ribeiro ; Mayer-Santos, Eric ; Eduardo, Carlos de Paula ; Freitas, Patricia Moreira de ; Aranha, Ana Cecilia Correa ; Moura-Netto, Cacio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-72d1abe980cd632537c3c9a7ad5f4535db937ebe6b00d50db540525174b92ec83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Dental Pulp Cavity - drug effects</topic><topic>Edetic Acid - therapeutic use</topic><topic>Endodontics</topic><topic>Humans</topic><topic>Lasers, Semiconductor</topic><topic>Methylene blue</topic><topic>Methylene Blue - adverse effects</topic><topic>Methylene Blue - therapeutic use</topic><topic>Photochemotherapy - methods</topic><topic>Photodynamic Therapy</topic><topic>Photosensitizing Agents - adverse effects</topic><topic>Photosensitizing Agents - therapeutic use</topic><topic>Random Allocation</topic><topic>Root Canal Irrigants - therapeutic use</topic><topic>Sodium Hypochlorite - therapeutic use</topic><topic>Tolonium Chloride - therapeutic use</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramalho, Karen Müller</creatorcontrib><creatorcontrib>Cunha, Sandra Ribeiro</creatorcontrib><creatorcontrib>Mayer-Santos, Eric</creatorcontrib><creatorcontrib>Eduardo, Carlos de Paula</creatorcontrib><creatorcontrib>Freitas, Patricia Moreira de</creatorcontrib><creatorcontrib>Aranha, Ana Cecilia Correa</creatorcontrib><creatorcontrib>Moura-Netto, Cacio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Photodiagnosis and photodynamic therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramalho, Karen Müller</au><au>Cunha, Sandra Ribeiro</au><au>Mayer-Santos, Eric</au><au>Eduardo, Carlos de Paula</au><au>Freitas, Patricia Moreira de</au><au>Aranha, Ana Cecilia Correa</au><au>Moura-Netto, Cacio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro evaluation of methylene blue removal from root canal after Photodynamic Therapy</atitle><jtitle>Photodiagnosis and photodynamic therapy</jtitle><addtitle>Photodiagnosis Photodyn Ther</addtitle><date>2017-12</date><risdate>2017</risdate><volume>20</volume><spage>248</spage><epage>252</epage><pages>248-252</pages><issn>1572-1000</issn><eissn>1873-1597</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>•The importance of cleaning the dentin after Photodynamic Therapy.•Test saline, NaOCl, EDTA and PUI to remove methylene blue of root canal.•No treatments completely remove methylene blue from the root canal after PDT.
In Endodontics, photosensitizers’ such as methylene blue and toluidine blue have been used in Photodynamic Therapy due to their positive results. However, they can stain the dentin from the root canal after Photodynamic Therapy (PDT). The present in vitro study aimed to evaluate different stain removal protocols from root canal after PDT using methylene blue (MB) dye. After mechanical preparation of the root canal of 40 uni-radicular human teeth, PDT was performed using 0,01% MB with parameters of 3 min of pre-irradiation and a diode laser irradiation emitting at 660 nm, 40 mW, 4 min, 9.6 J. After PDT, different protocols of MB removal were performed: Group 1 − control (0.9% saline solution); Group 2 − sodium hypochlorite (2.5% NaOCl); Group 3–17% ethylenediamine tetraacetic acid (EDTA); Group 4 − passive ultrasonic irrigation (PUI); The color of the dentin of the root canal was measured, before, immediately after the PDT and immediately after the cleaning using a spectrophotometer. The ΔE values found were statistically compared using the ANOVA and Tukey's tests (α=0.05). All the treatments lead to some cleaning of root canal after PDT, however, none of the treatments tested completely removed all staining caused by MB photosensitizer of the root canal. Among the treatments tested, PUI and Hypochlorite 2.5% promoted greater cleaning, with no statistically significant difference between them. In conclusion, within the protocols tested in the present study, no treatments were able to completely remove MB staining of the root canal after PDT.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>29097290</pmid><doi>10.1016/j.pdpdt.2017.10.024</doi><tpages>5</tpages></addata></record> |
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| ispartof | Photodiagnosis and photodynamic therapy, 2017-12, Vol.20, p.248-252 |
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| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Dental Pulp Cavity - drug effects Edetic Acid - therapeutic use Endodontics Humans Lasers, Semiconductor Methylene blue Methylene Blue - adverse effects Methylene Blue - therapeutic use Photochemotherapy - methods Photodynamic Therapy Photosensitizing Agents - adverse effects Photosensitizing Agents - therapeutic use Random Allocation Root Canal Irrigants - therapeutic use Sodium Hypochlorite - therapeutic use Tolonium Chloride - therapeutic use |
| title | In vitro evaluation of methylene blue removal from root canal after Photodynamic Therapy |
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