In vitro evaluation of methylene blue removal from root canal after Photodynamic Therapy

•The importance of cleaning the dentin after Photodynamic Therapy.•Test saline, NaOCl, EDTA and PUI to remove methylene blue of root canal.•No treatments completely remove methylene blue from the root canal after PDT. In Endodontics, photosensitizers’ such as methylene blue and toluidine blue have b...

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Published in:Photodiagnosis and photodynamic therapy 2017-12, Vol.20, p.248-252
Main Authors: Ramalho, Karen Müller, Cunha, Sandra Ribeiro, Mayer-Santos, Eric, Eduardo, Carlos de Paula, Freitas, Patricia Moreira de, Aranha, Ana Cecilia Correa, Moura-Netto, Cacio
Format: Article
Language:English
Subjects:
Dental Pulp Cavity - drug effects
Edetic Acid - therapeutic use
Endodontics
Humans
Lasers, Semiconductor
Methylene blue
Methylene Blue - adverse effects
Methylene Blue - therapeutic use
Photochemotherapy - methods
Photodynamic Therapy
Photosensitizing Agents - adverse effects
Photosensitizing Agents - therapeutic use
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Root Canal Irrigants - therapeutic use
Sodium Hypochlorite - therapeutic use
Tolonium Chloride - therapeutic use
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Summary:•The importance of cleaning the dentin after Photodynamic Therapy.•Test saline, NaOCl, EDTA and PUI to remove methylene blue of root canal.•No treatments completely remove methylene blue from the root canal after PDT. In Endodontics, photosensitizers’ such as methylene blue and toluidine blue have been used in Photodynamic Therapy due to their positive results. However, they can stain the dentin from the root canal after Photodynamic Therapy (PDT). The present in vitro study aimed to evaluate different stain removal protocols from root canal after PDT using methylene blue (MB) dye. After mechanical preparation of the root canal of 40 uni-radicular human teeth, PDT was performed using 0,01% MB with parameters of 3 min of pre-irradiation and a diode laser irradiation emitting at 660 nm, 40 mW, 4 min, 9.6 J. After PDT, different protocols of MB removal were performed: Group 1 − control (0.9% saline solution); Group 2 − sodium hypochlorite (2.5% NaOCl); Group 3–17% ethylenediamine tetraacetic acid (EDTA); Group 4 − passive ultrasonic irrigation (PUI); The color of the dentin of the root canal was measured, before, immediately after the PDT and immediately after the cleaning using a spectrophotometer. The ΔE values found were statistically compared using the ANOVA and Tukey's tests (α=0.05). All the treatments lead to some cleaning of root canal after PDT, however, none of the treatments tested completely removed all staining caused by MB photosensitizer of the root canal. Among the treatments tested, PUI and Hypochlorite 2.5% promoted greater cleaning, with no statistically significant difference between them. In conclusion, within the protocols tested in the present study, no treatments were able to completely remove MB staining of the root canal after PDT.
ISSN:1572-1000
1873-1597
DOI:10.1016/j.pdpdt.2017.10.024