rpoB gene sequencing highlights the prevalence of an E. miricola cluster over other Elizabethkingia species among UK cystic fibrosis patients

Difficulties in distinguishing species of the Elizabethkingia genus by MALDI-TOF prompted use of rpoB sequencing to investigate species distribution among 44 isolates from cystic fibrosis (CF) patients. Forty-three isolates from 38 patients formed a cluster comprising E. miricola and proposed novel...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2018-02, Vol.90 (2), p.109-114
Main Authors: Kenna, Dervla T.D., Fuller, Alice, Martin, Kate, Perry, Claire, Pike, Rachel, Burns, Phillipa J., Narayan, Omendra, Wilkinson, Stuart, Hill, Robert, Woodford, Neil, Logan, Julie M.J., Turton, Jane F.
Format: Article
Language:English
Subjects:
Cystic fibrosis
Elizabethkingia spp
Minimum inhibitory concentration
Prevalence
Urease
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Summary:Difficulties in distinguishing species of the Elizabethkingia genus by MALDI-TOF prompted use of rpoB sequencing to investigate species distribution among 44 isolates from cystic fibrosis (CF) patients. Forty-three isolates from 38 patients formed a cluster comprising E. miricola and proposed novel species E. bruuniana sp. nov., the exception clustering with proposed species E. ursingii sp. nov., also part of this wider cluster. All 44 isolates were PCR-positive for urease gene ureG, whereas only one of 23 E. anophelis isolates from non-CF patients was positive, suggesting that this gene is largely associated with the E. miricola cluster. Antibiotic susceptibilities of 12 CF isolates revealed all were resistant to beta-lactams with the exception of piperacillin-tazobactam, and were only susceptible to minocycline and co-trimoxazole. Pulsed-field gel electrophoresis analysis revealed 4 shared strains among 17 CF patients in one pediatric clinic, but epidemiological investigations did not support patient-to-patient transmission except between one sibling pair. •Speciation within the Elizabethkingia genus is difficult using conventional methods•rpoB sequencing separated current species into 3 clearly defined clusters•Forty-four cystic fibrosis patient isolates belonged to an E. miricola cluster•All 44 isolates and one E. anophelis isolate were PCR-positive for urease gene ureG•Twelve CF isolates were resistant to beta-lactams except piperacillin-tazobactam
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2017.10.014