Nuclear myosin 1 associates with papillomavirus E2 regulatory protein and influences viral replication

Nuclear myosin 1c (NM1) associates with RNA polymerases and is a partner in the chromatin remodeling complex B-WICH. This complex, which also contains WSTF and SNF2h proteins, is involved in transcriptional regulation. We report herein that papillomavirus protein E2 binds to NM1 and co-precipitates...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2018-01, Vol.514, p.142-155
Main Authors: Sankovski, Eve, Abroi, Aare, Ustav, Mart
Format: Article
Language:English
Subjects:
E2 protein
Nuclear myosin I
Papillomavirus
Viral replication
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Summary:Nuclear myosin 1c (NM1) associates with RNA polymerases and is a partner in the chromatin remodeling complex B-WICH. This complex, which also contains WSTF and SNF2h proteins, is involved in transcriptional regulation. We report herein that papillomavirus protein E2 binds to NM1 and co-precipitates with the WSTF and SNF2h proteins. Our data suggest that E2 associates with the cellular B-WICH complex through binding to NM1. E2 and NM1 associate via their N-terminal domains and this interaction is ATP dependent. The cellular multifunctional protein Brd4 and beta-actin are also present in the NM1-E2 complex. NM1 downregulation by siRNA increases the replication of the BPV1 and HPV5 genomes but does not affect HPV18 genome replication. These results suggest that the B-WICH complex may play a role in the papillomavirus life cycle through NM1 and E2 protein interaction. •We have discovered interaction of papillomavirus E2 protein with the nuclear myosin I (NM1) complex in eucaryotic cells.•This highlights complexity of interaction of the viral transcription and replication factor with the host cell proteins.•NM1 is involved in replication of several papillomavirus genomes.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2017.11.013