Liposomally formulated phospholipid-conjugated novel near-infrared fluorescence probe for particle size effect on cellular uptake and biodistribution in vivo

[Display omitted] •iDSPE was a novel near-infrared probe with high fluorescence.•iDSPE was conjugated by DSPE and indocyanine green derivative ir623 via covalent bond.•Smaller iDSPE-embeded liposomes (iLPs) (∼100nm) were found to be taken up in greater quantities by HT29 cells.•Small iLPs was mainly...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2018-01, Vol.161, p.588-596
Main Authors: Xing, Jing, Liu, Dong, Zhou, Gaoxin, Li, Yuan, Wang, Peng, Hu, Ke, Gu, Ning, Ji, Min
Format: Article
Language:English
Subjects:
Biodistribution
Cellular uptake
Lipid based nanoparticles
Near-infrared fluorescence probe
Particle size
Phospholipid-conjugated
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Summary:[Display omitted] •iDSPE was a novel near-infrared probe with high fluorescence.•iDSPE was conjugated by DSPE and indocyanine green derivative ir623 via covalent bond.•Smaller iDSPE-embeded liposomes (iLPs) (∼100nm) were found to be taken up in greater quantities by HT29 cells.•Small iLPs was mainly accumulated in the tumor tissues; larger one (∼400nm) was accumulated in liver and spleen. Lipid based nanoparticles (LBNs) with excellent biocompatibility and versatility have received much attention from the drug delivery community recently. A detailed understanding of in vitro and vivo fate of LBNs is important for developing different types of LBNs with improved selectivity and low cytotoxicity. We developed a novel near-infrared (NIR) probe with high fluorescence, designated as DSPE-ir623 (iDSPE). Then, we prepared iDSPE-embeded liposomes (iLPs) with two different hydrodynamic sizes (∼100nm and ∼400nm) to evaluate the effect of particle size on cellular uptake and biodistribution of nanoliposomes in vivo. These iLPs were proved to exhibit good monodispersity, excellent fluorescence and stability. In vitro cell uptake tests demonstrated that iLPs-1 (∼100nm) were taken up more by HT-29 cells than iLPs-2 (∼400nm). Notably, the fluorescence of iLPs can be employed for real-time monitoring of the subcellular locating and its metabolic distribution in vivo. Near-infrared imaging in vivo illustrated that iLPs-1 was mainly accumulated in the tumor tissues, while iLPs-2 was accumulated in liver and spleen. The results indicated that the size of iLPs play an important role in the regulation of intracellular trafficking and biodistribution of liposomes, which also provide a new insight into the development of more effective LBNs. Hence, iDSPE might be a promising tool for the reliable tracing of different types of LBNs.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2017.11.033