TGF-β signaling regulates DACT1 expression in intestinal epithelial cells

[Display omitted] DACT1, was first identified as a Dishevelled-associated antagonist of Wnt signaling pathway. It has been reported that DACT1 functions in embryonic development and tumorigenesis. However, the regulation of DACT1 still remains unclear. We found Wnt signaling has no effect on DACT1,...

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Bibliographic Details
Published in:Biomedicine & pharmacotherapy 2018-01, Vol.97, p.864-869
Main Authors: Wang, Ji-lian, Zhou, Xin, Zhang, Ling-fu, Li, Fei, Wang, Bing-yan, Wang, Wen-dong, Fu, Wei
Format: Article
Language:English
Subjects:
DACT1
TGF-β
Transcriptional regulation
Wnt signaling
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Summary:[Display omitted] DACT1, was first identified as a Dishevelled-associated antagonist of Wnt signaling pathway. It has been reported that DACT1 functions in embryonic development and tumorigenesis. However, the regulation of DACT1 still remains unclear. We found Wnt signaling has no effect on DACT1, but TGF-β increases expression of DACT1 in intestinal epithelial cells. In addition, the minimal promoter is located in the region of −500bp to +1bp and the region between −3000bp to +1bp enhanced promoter activity. Site-directed mutation analysis was performed and indicated that potential regulatory elements was near −335bp. Our study provided the basic information for the exploration of DACT1 regulation and expression. Moreover, TGF-β inhibits Wnt signaling to enhance the function of DACT1 inhibiting Wnt signaling.
ISSN:0753-3322
1950-6007
DOI:10.1016/j.biopha.2017.11.017