Desensitization of cold-and menthol-sensitive rat dorsal root ganglion neurones by inflammatory mediators

The interaction between cold sensitivity and inflammation in mammals is not entirely understood. We have used adult rat dorsal root ganglion neurones in primary culture together with calcium microfluorimetry to assess the effects of selected inflammatory mediators on cold responses of cold- and ment...

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Bibliographic Details
Published in:Experimental brain research 2007-03, Vol.178 (1), p.89-98
Main Authors: LINTE, Ramona Madalina, CIOBANU, Cristian, REID, Gordon, BABES, Alexandru
Format: Article
Language:English
Subjects:
Analgesia - methods
Animals
Ankyrins
Antipruritics - pharmacology
Biological and medical sciences
Calcium Channels - drug effects
Calcium Channels - metabolism
Cells, Cultured
Cold Temperature
Cooling
Cranial nerves. Spinal roots. Peripheral nerves. Autonomic nervous system. Gustation. Olfaction
Cyclic AMP-Dependent Protein Kinases - metabolism
Fundamental and applied biological sciences. Psychology
Ganglia, Spinal - physiology
Heat
Histamine
Hyperalgesia
Inflammation - chemically induced
Inflammation - physiopathology
Inflammation Mediators - pharmacology
Kinases
Medical sciences
Menthol - pharmacology
Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration
Nervous system (semeiology, syndromes)
Neurology
Neurons, Afferent - physiology
Nociceptors - drug effects
Nociceptors - physiology
Pain - chemically induced
Pain - physiopathology
Physiology
Protein Kinase C - metabolism
Proteins
Rats
Rats, Wistar
Reaction Time - physiology
Sensory Thresholds - drug effects
Sensory Thresholds - physiology
Thermosensing - drug effects
Thermosensing - physiology
TRPA1 Cation Channel
TRPC Cation Channels
TRPM Cation Channels - drug effects
TRPM Cation Channels - metabolism
Vertebrates: nervous system and sense organs
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Summary:The interaction between cold sensitivity and inflammation in mammals is not entirely understood. We have used adult rat dorsal root ganglion neurones in primary culture together with calcium microfluorimetry to assess the effects of selected inflammatory mediators on cold responses of cold- and menthol-sensitive (most likely TRPM8-expressing) neurones. We observed a high degree of functional co-expression of TRPM8, the receptors for the inflammatory agents bradykinin, prostaglandin E2 and histamine, and TRPA1 in cultured sensory neurones. Treatment with either bradykinin or prostaglandin E2 led to a reduction in the amplitude of the response to cooling and shifted the threshold temperature to colder values, and we provide evidence for a role of protein kinases C and A, respectively, in mediating these effects. In both cases the effects were mainly restricted to the subgroups of cold- and menthol-sensitive cells which had responded to the application of the inflammatory agents at basal temperature. This desensitization of cold-sensitive neurones may enhance inflammatory pain by removing the analgesic effects of gentle cooling.
ISSN:0014-4819
1432-1106
DOI:10.1007/s00221-006-0712-3