Temperature cycling during platelet cold storage improves in vivo recovery and survival in healthy volunteers

BACKGROUND Room temperature (RT) storage of platelets (PLTs) can support bacterial proliferation in contaminated units, which can lead to transfusion‐transmitted septic reactions. Cold temperature storage of PLTs could reduce bacterial proliferation but cold exposure produces activation‐like changes...

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Bibliographic Details
Published in:Transfusion (Philadelphia, Pa.) Pa.), 2018-01, Vol.58 (1), p.25-33
Main Authors: Vostal, Jaroslav G., Gelderman, Monique P., Skripchenko, Andrey, Xu, Fei, Li, Ying, Ryan, Johannah, Cheng, Chunrong, Whitley, Pam, Wellington, Michael, Sawyer, Sherrie, Hanley, Shalene, Wagner, Stephen J.
Format: Article
Language:English
Subjects:
Apheresis
Bacteria
Cold storage
Cycles
Kinetics
Platelets
Reaction kinetics
Recovery
Survival
Temperature effects
Transfusion
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Summary:BACKGROUND Room temperature (RT) storage of platelets (PLTs) can support bacterial proliferation in contaminated units, which can lead to transfusion‐transmitted septic reactions. Cold temperature storage of PLTs could reduce bacterial proliferation but cold exposure produces activation‐like changes in PLTs and leads to their rapid clearance from circulation. Cold‐induced changes are reversible by warming and periodic rewarming during cold storage (temperature cycling [TC]) has been proposed to alleviate cold‐induced reduction in PLT circulation. STUDY DESIGN AND METHODS A clinical trial in healthy human volunteers was designed to compare in vivo recovery, survival, and area under the curve (AUC) of radiolabeled autologous apheresis PLTs stored for 7 days at RT or under TC or cold conditions. Paired comparisons of RT versus TC and TC versus cold PLTs were conducted. RESULTS Room temperature PLTs had in vivo recovery of 55.7 ± 13.9%, survival of 161.3 ± 28.8 hours, and AUC of 5031.2 ± 1643.3. TC PLTs had recovery of 42.6 ± 16.4%, survival of 48.1 ± 14.4% hours, and AUC of 1331.3 ± 910.2 (n = 12, p 
ISSN:0041-1132
1537-2995
DOI:10.1111/trf.14392