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Spectromicroscopic observation of a live single cell in a biocompatible liquid-enclosing graphene system
On-the-spot visualization of biochemical responses of intact live cells is vital for a clear understanding of cell biology. The main obstacles for instant visualization of biochemical responses of living cells arise from the lack of a sophisticated detecting technique which can simultaneously provid...
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| Published in: | Nanoscale 2018-01, Vol.10 (1), p.150-157 |
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| Main Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c315t-ad853e717818f734c8d2447a3061f49603832beaf8c94f06e03ce881830aa1393 |
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| cites | cdi_FETCH-LOGICAL-c315t-ad853e717818f734c8d2447a3061f49603832beaf8c94f06e03ce881830aa1393 |
| container_end_page | 157 |
| container_issue | 1 |
| container_start_page | 150 |
| container_title | Nanoscale |
| container_volume | 10 |
| creator | Yu, Seong Uk Lee, Hwiwon Cho, Woo Jong Kim, Chulhyun Kang, Moon Cheol Shin, Hyun-Joon Kim, Namdong Hahn, Sei Kwang Kim, Kwang S |
| description | On-the-spot visualization of biochemical responses of intact live cells is vital for a clear understanding of cell biology. The main obstacles for instant visualization of biochemical responses of living cells arise from the lack of a sophisticated detecting technique which can simultaneously provide chemical analysis tools and the biocompatible wet conditions. Here we introduce scanning transmission X-ray microscopy (STXM) combined with a liquid-enclosing graphene system (LGS), offering biocompatible conditions and improved X-ray absorption spectra to probe the chemical responses of live cells under wet conditions. This set-up enables us to probe a subtle change in absorption spectra depending on the oxidation state of a miniscule amount of oxygen in the functional groups present in each cell and its surroundings containing a minimal amount of liquid water. As an example of in situ biochemical responses of wet cells, chemical responses of a single Colo 205 cell are visualized and analyzed using X-ray absorption near the oxygen K-edge. This spectromicroscopic method using LGS can be applied to diverse biological samples under wet conditions for the analysis of their biochemical responses. |
| doi_str_mv | 10.1039/c7nr05223e |
| format | article |
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The main obstacles for instant visualization of biochemical responses of living cells arise from the lack of a sophisticated detecting technique which can simultaneously provide chemical analysis tools and the biocompatible wet conditions. Here we introduce scanning transmission X-ray microscopy (STXM) combined with a liquid-enclosing graphene system (LGS), offering biocompatible conditions and improved X-ray absorption spectra to probe the chemical responses of live cells under wet conditions. This set-up enables us to probe a subtle change in absorption spectra depending on the oxidation state of a miniscule amount of oxygen in the functional groups present in each cell and its surroundings containing a minimal amount of liquid water. As an example of in situ biochemical responses of wet cells, chemical responses of a single Colo 205 cell are visualized and analyzed using X-ray absorption near the oxygen K-edge. This spectromicroscopic method using LGS can be applied to diverse biological samples under wet conditions for the analysis of their biochemical responses.</description><identifier>ISSN: 2040-3364</identifier><identifier>EISSN: 2040-3372</identifier><identifier>DOI: 10.1039/c7nr05223e</identifier><identifier>PMID: 29115327</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Absorption spectra ; Biochemistry ; Biocompatibility ; Biocompatible Materials ; Biological properties ; Cell Line ; Chemical analysis ; Functional groups ; Graphene ; Graphite - chemistry ; Humans ; Microscopy - methods ; Microscopy, Confocal ; Oxidation ; Oxidation-Reduction ; Oxygen ; Shellfish ; Spectrum Analysis - methods ; Valence ; Visualization ; Water ; Wet cells ; X ray microscopy ; X-Rays</subject><ispartof>Nanoscale, 2018-01, Vol.10 (1), p.150-157</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c315t-ad853e717818f734c8d2447a3061f49603832beaf8c94f06e03ce881830aa1393</citedby><cites>FETCH-LOGICAL-c315t-ad853e717818f734c8d2447a3061f49603832beaf8c94f06e03ce881830aa1393</cites><orcidid>0000-0002-7718-6259 ; 0000-0002-6929-5359</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29115327$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Seong Uk</creatorcontrib><creatorcontrib>Lee, Hwiwon</creatorcontrib><creatorcontrib>Cho, Woo Jong</creatorcontrib><creatorcontrib>Kim, Chulhyun</creatorcontrib><creatorcontrib>Kang, Moon Cheol</creatorcontrib><creatorcontrib>Shin, Hyun-Joon</creatorcontrib><creatorcontrib>Kim, Namdong</creatorcontrib><creatorcontrib>Hahn, Sei Kwang</creatorcontrib><creatorcontrib>Kim, Kwang S</creatorcontrib><title>Spectromicroscopic observation of a live single cell in a biocompatible liquid-enclosing graphene system</title><title>Nanoscale</title><addtitle>Nanoscale</addtitle><description>On-the-spot visualization of biochemical responses of intact live cells is vital for a clear understanding of cell biology. The main obstacles for instant visualization of biochemical responses of living cells arise from the lack of a sophisticated detecting technique which can simultaneously provide chemical analysis tools and the biocompatible wet conditions. Here we introduce scanning transmission X-ray microscopy (STXM) combined with a liquid-enclosing graphene system (LGS), offering biocompatible conditions and improved X-ray absorption spectra to probe the chemical responses of live cells under wet conditions. This set-up enables us to probe a subtle change in absorption spectra depending on the oxidation state of a miniscule amount of oxygen in the functional groups present in each cell and its surroundings containing a minimal amount of liquid water. As an example of in situ biochemical responses of wet cells, chemical responses of a single Colo 205 cell are visualized and analyzed using X-ray absorption near the oxygen K-edge. This spectromicroscopic method using LGS can be applied to diverse biological samples under wet conditions for the analysis of their biochemical responses.</description><subject>Absorption spectra</subject><subject>Biochemistry</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials</subject><subject>Biological properties</subject><subject>Cell Line</subject><subject>Chemical analysis</subject><subject>Functional groups</subject><subject>Graphene</subject><subject>Graphite - chemistry</subject><subject>Humans</subject><subject>Microscopy - methods</subject><subject>Microscopy, Confocal</subject><subject>Oxidation</subject><subject>Oxidation-Reduction</subject><subject>Oxygen</subject><subject>Shellfish</subject><subject>Spectrum Analysis - methods</subject><subject>Valence</subject><subject>Visualization</subject><subject>Water</subject><subject>Wet cells</subject><subject>X ray microscopy</subject><subject>X-Rays</subject><issn>2040-3364</issn><issn>2040-3372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpd0E1Lw0AQBuBFFFurF3-ABLyIEN3dSbKbo5T6AaLgxzlstpN2S5JNd5NC_72JrT14mmF4ZhheQi4ZvWMU0nstakdjzgGPyJjTiIYAgh8f-iQakTPvV5QmKSRwSkY8ZSwGLsZk-dmgbp2tjHbWa9sYHdjco9uo1tg6sEWggtJsMPCmXpQYaCzLwNT9NDdW26rpXd7PS7PuzDzEWpd2oMHCqWaJdb-49S1W5-SkUKXHi32dkO_H2df0OXx9f3qZPryGGljchmouY0DBhGSyEBBpOedRJBTQhBVRmlCQwHNUhdRpVNAEKWiUPQaqFIMUJuRmd7dxdt2hb7PK-OFpVaPtfMbShMlYCh719PofXdnO1f13GaeMShFzNqjbnRoC8g6LrHGmUm6bMZoN-WdT8fbxm_-sx1f7k11e4fxA_wKHH_0tgBg</recordid><startdate>20180107</startdate><enddate>20180107</enddate><creator>Yu, Seong Uk</creator><creator>Lee, Hwiwon</creator><creator>Cho, Woo Jong</creator><creator>Kim, Chulhyun</creator><creator>Kang, Moon Cheol</creator><creator>Shin, Hyun-Joon</creator><creator>Kim, Namdong</creator><creator>Hahn, Sei Kwang</creator><creator>Kim, Kwang S</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7718-6259</orcidid><orcidid>https://orcid.org/0000-0002-6929-5359</orcidid></search><sort><creationdate>20180107</creationdate><title>Spectromicroscopic observation of a live single cell in a biocompatible liquid-enclosing graphene system</title><author>Yu, Seong Uk ; 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The main obstacles for instant visualization of biochemical responses of living cells arise from the lack of a sophisticated detecting technique which can simultaneously provide chemical analysis tools and the biocompatible wet conditions. Here we introduce scanning transmission X-ray microscopy (STXM) combined with a liquid-enclosing graphene system (LGS), offering biocompatible conditions and improved X-ray absorption spectra to probe the chemical responses of live cells under wet conditions. This set-up enables us to probe a subtle change in absorption spectra depending on the oxidation state of a miniscule amount of oxygen in the functional groups present in each cell and its surroundings containing a minimal amount of liquid water. As an example of in situ biochemical responses of wet cells, chemical responses of a single Colo 205 cell are visualized and analyzed using X-ray absorption near the oxygen K-edge. 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| ispartof | Nanoscale, 2018-01, Vol.10 (1), p.150-157 |
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| source | Royal Society of Chemistry:Jisc Collections:Royal Society of Chemistry Read and Publish 2022-2024 (reading list) |
| subjects | Absorption spectra Biochemistry Biocompatibility Biocompatible Materials Biological properties Cell Line Chemical analysis Functional groups Graphene Graphite - chemistry Humans Microscopy - methods Microscopy, Confocal Oxidation Oxidation-Reduction Oxygen Shellfish Spectrum Analysis - methods Valence Visualization Water Wet cells X ray microscopy X-Rays |
| title | Spectromicroscopic observation of a live single cell in a biocompatible liquid-enclosing graphene system |
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