Spectromicroscopic observation of a live single cell in a biocompatible liquid-enclosing graphene system

On-the-spot visualization of biochemical responses of intact live cells is vital for a clear understanding of cell biology. The main obstacles for instant visualization of biochemical responses of living cells arise from the lack of a sophisticated detecting technique which can simultaneously provid...

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Bibliographic Details
Published in:Nanoscale 2018-01, Vol.10 (1), p.150-157
Main Authors: Yu, Seong Uk, Lee, Hwiwon, Cho, Woo Jong, Kim, Chulhyun, Kang, Moon Cheol, Shin, Hyun-Joon, Kim, Namdong, Hahn, Sei Kwang, Kim, Kwang S
Format: Article
Language:English
Subjects:
Absorption spectra
Biochemistry
Biocompatibility
Biocompatible Materials
Biological properties
Cell Line
Chemical analysis
Functional groups
Graphene
Graphite - chemistry
Humans
Microscopy - methods
Microscopy, Confocal
Oxidation
Oxidation-Reduction
Oxygen
Shellfish
Spectrum Analysis - methods
Valence
Visualization
Water
Wet cells
X ray microscopy
X-Rays
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Summary:On-the-spot visualization of biochemical responses of intact live cells is vital for a clear understanding of cell biology. The main obstacles for instant visualization of biochemical responses of living cells arise from the lack of a sophisticated detecting technique which can simultaneously provide chemical analysis tools and the biocompatible wet conditions. Here we introduce scanning transmission X-ray microscopy (STXM) combined with a liquid-enclosing graphene system (LGS), offering biocompatible conditions and improved X-ray absorption spectra to probe the chemical responses of live cells under wet conditions. This set-up enables us to probe a subtle change in absorption spectra depending on the oxidation state of a miniscule amount of oxygen in the functional groups present in each cell and its surroundings containing a minimal amount of liquid water. As an example of in situ biochemical responses of wet cells, chemical responses of a single Colo 205 cell are visualized and analyzed using X-ray absorption near the oxygen K-edge. This spectromicroscopic method using LGS can be applied to diverse biological samples under wet conditions for the analysis of their biochemical responses.
ISSN:2040-3364
2040-3372
DOI:10.1039/c7nr05223e