Neutron Reflectometry Elucidates Protein Adsorption from Human Blood Serum onto PEG Brushes

Poly­(ethylene glycol) (PEG) brushes are reputed for their ability to prevent undesired protein adsorption to material surfaces exposed to biological fluids. Here, protein adsorption out of human blood serum onto PEG brushes anchored to solid-supported lipid monolayers was characterized by neutron r...

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Bibliographic Details
Published in:Langmuir 2017-11, Vol.33 (44), p.12708-12718
Main Authors: Latza, Victoria M, Rodriguez-Loureiro, Ignacio, Kiesel, Irena, Halperin, Avraham, Fragneto, Giovanna, Schneck, Emanuel
Format: Article
Language:English
Subjects:
Adsorption
Blood Proteins
Humans
Neutrons
Polyethylene Glycols
Surface Properties
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Summary:Poly­(ethylene glycol) (PEG) brushes are reputed for their ability to prevent undesired protein adsorption to material surfaces exposed to biological fluids. Here, protein adsorption out of human blood serum onto PEG brushes anchored to solid-supported lipid monolayers was characterized by neutron reflectometry, yielding volume fraction profiles of lipid headgroups, PEG, and adsorbed proteins at subnanometer resolution. For both PEGylated and non-PEGylated lipid surfaces, serum proteins adsorb as a thin layer of approximately 10 Å, overlapping with the headgroup region. This layer corresponds to primary adsorption at the grafting surface and resists rinsing. A second diffuse protein layer overlaps with the periphery of the PEG brush and is attributed to ternary adsorption due to protein–PEG attraction. This second layer disappears upon rinsing, thus providing a first observation of the structural effect of rinsing on protein adsorption to PEG brushes.
ISSN:0743-7463
1520-5827
DOI:10.1021/acs.langmuir.7b03048