A lncRNA-H19 transcript from secondary hair follicle of Liaoning cashmere goat: Identification, regulatory network and expression regulated potentially by its promoter methylation

The H19 transcript (imprinted maternally expressed transcript) is well-known as long noncoding RNA (lncRNA), and it is thought to be associated with the inductive capacity of dermal papilla cells for hair-follicle reconstruction. In this study, we isolated and characterized a lncRNA-H19 transcript f...

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Bibliographic Details
Published in:Gene 2018-01, Vol.641, p.78-85
Main Authors: Zhu, Yu B., Wang, Ze Y., Yin, Rong H., Jiao, Qian, Zhao, Su J., Cong, Yu Y., Xue, Hui L., Guo, Dan, Wang, Shi Q., Zhu, Yan X., Bai, Wen L.
Format: Article
Language:English
Subjects:
Animal Fur - cytology
Animals
Base Composition - genetics
Base Sequence
DNA Methylation - genetics
Expression patterns
Gene Expression Regulation - genetics
Goats - genetics
Hair Follicle - cytology
Liaoning cashmere goat
lncRNA-H19
Promoter methylation
Promoter Regions, Genetic - genetics
Regulatory network
RNA, Long Noncoding - genetics
Secondary hair follicle
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Summary:The H19 transcript (imprinted maternally expressed transcript) is well-known as long noncoding RNA (lncRNA), and it is thought to be associated with the inductive capacity of dermal papilla cells for hair-follicle reconstruction. In this study, we isolated and characterized a lncRNA-H19 transcript from the secondary hair follicle of Liaoning cashmere goat. Also, we investigated its transcriptional pattern and methylation status of H19 gene in secondary hair follicle of this breed during different stages of hair follicle cycle. Nucleotide composition analysis indicated that guanine (G) and cytosine (C) are the dominant nucleotides in the lncRNA-H19 transcript of Liaoning cashmere goat with the highest frequency distribution (11.25%) of GG nucleotide pair. The regulatory network showed that lncRNA-H19 transcript appears to have remarkably diverse regulatory relationships with its related miRNAs and the potential target genes. In secondary hair follicle, the relative expression of lncRNA-H19 transcript at the anagen phase is significantly higher than that at both telogen and catagen phases suggesting that lncRNA-H19 transcript might play essential roles in the formation and growth of cashmere fiber of goat. Methylation analysis indicated that the methylation of the promoter region of H19 gene most likely participates in its transcriptional suppression in secondary hair follicle of Liaoning cashmere goat. •Guanine and cytosine are dominant nucleotides in lncRNA-H19 of Liaoning cashmere goat.•LncRNA-H19 have diverse regulatory relationships with related miRNAs and target genes.•LncRNA-H19 exhibits higher expression in secondary hair follicle at anagen than at catagen and telogen.•Promoter methylation of H19 gene might participate in its transcriptional suppression in secondary hair follicle.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2017.10.028