Melatonin reduces oxidative damage and upregulates heat shock protein 90 expression in cryopreserved human semen

Sperm cells can be damaged during the semen cryopreservation process, decreasing their fertilizing ability. Physical damage and oxidative stress may occur during the freeze–thawing process. Antioxidants such as the native antioxidant melatonin can potentially improve cryopreservation outcomes. In th...

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Bibliographic Details
Published in:Free radical biology & medicine 2017-12, Vol.113, p.347-354
Main Authors: Deng, Shou-Long, Sun, Tie-Cheng, Yu, Kun, Wang, Zhi-Peng, Zhang, Bao-Lu, Zhang, Yan, Wang, Xiu-Xia, Lian, Zheng-Xing, Liu, Yi-Xun
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - biosynthesis
Antioxidants - pharmacology
Benzoquinones - pharmacology
Cell Survival - drug effects
Cryopreservation - methods
Cryoprotective Agents - pharmacology
Gene Expression Regulation - drug effects
HSP90
HSP90 Heat-Shock Proteins - agonists
HSP90 Heat-Shock Proteins - antagonists & inhibitors
HSP90 Heat-Shock Proteins - genetics
HSP90 Heat-Shock Proteins - metabolism
Human
Humans
Lactams, Macrocyclic - pharmacology
Lipid Peroxidation - drug effects
Male
Melatonin
Melatonin - pharmacology
NF-E2-Related Factor 2 - agonists
NF-E2-Related Factor 2 - genetics
NF-E2-Related Factor 2 - metabolism
Oxidative stress
Oxidative Stress - drug effects
Protein Biosynthesis
Proto-Oncogene Proteins c-bcl-2 - agonists
Proto-Oncogene Proteins c-bcl-2 - genetics
Proto-Oncogene Proteins c-bcl-2 - metabolism
Reactive Oxygen Species - antagonists & inhibitors
Reactive Oxygen Species - metabolism
Receptor, Melatonin, MT1 - antagonists & inhibitors
Receptor, Melatonin, MT1 - genetics
Receptor, Melatonin, MT1 - metabolism
Semen - cytology
Semen - drug effects
Semen - metabolism
Semen Analysis
Semen cryopreservation
Sperm Motility - drug effects
Tetrahydronaphthalenes - pharmacology
Tryptamines - pharmacology
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Summary:Sperm cells can be damaged during the semen cryopreservation process, decreasing their fertilizing ability. Physical damage and oxidative stress may occur during the freeze–thawing process. Antioxidants such as the native antioxidant melatonin can potentially improve cryopreservation outcomes. In this study, we added melatonin to cryoprotectant to examine its effect on frozen–thawed human sperm. We found that adding 0.1mM melatonin to cryoprotectant significantly increased sperm viability (24.80 ± 0.46% vs. 20.97 ± 1.27%, P < 0.05) and membrane integrity (P < 0.05), and decreased intracellular reactive oxygen species and lipid peroxidation damage. Furthermore, mRNA levels of the transcription factor NF-E2-related factor-2 and its downstream genes were significantly increased. Resistance to oxidative stress was enhanced and expression of the antiapoptotic gene Bcl-2 was increased by inclusion of 0.1mM melatonin in the cryoprotectant. Moreover, 0.1mM melatonin upregulated the expression of heat shock protein 90 (HSP90), which confers resistance to stressors in frozen–thawed sperm. Results obtained upon addition of inhibitors of melatonin receptors (luzindole and 4-P-PDOT) and an HSP90 inhibitor (geldanamycin) in the cryoprotectant demonstrated that melatonin promoted HSP90 translation via the melatonin receptor MT1 and increased adenosine triphosphate levels, thus increasing the viability of thawed sperm. •Melatonin increases motility and membrane integrity of frozen–thawed sperm.•Melatonin decreases the ROS content of frozen–thawed human sperm.•Melatonin promotes HSP90 expression in frozen–thawed human sperm via MT1.
ISSN:0891-5849
1873-4596
DOI:10.1016/j.freeradbiomed.2017.10.342