Anxiolytic effect of fatty acids and terpenes fraction from Aloysia triphylla: Serotoninergic, GABAergic and glutamatergic implications

Aloysia triphylla (Verbenaceae) is an aromatic medicinal plant, and it is used for the treatment of “nervous” problems as, “sadness” and “nervousness”. While, there are no reports about its pharmacological activity in animal models. The objective of this work was to evaluate the anxiolytic effect of...

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Bibliographic Details
Published in:Biomedicine & pharmacotherapy 2017-12, Vol.96, p.320-327
Main Authors: Jiménez-Ferrer, Enrique, Santillán-Urquiza, Mayra Alejandra, Alegría-Herrera, Elian, Zamilpa, Alejandro, Noguerón-Merino, Carmen, Tortoriello, Jaime, Navarro-García, Victor, Avilés-Flores, Margarita, Fuentes-Mata, Macrina, Herrera-Ruiz, Maribel
Format: Article
Language:English
Subjects:
Animals
Anti-Anxiety Agents - isolation & purification
Anti-Anxiety Agents - pharmacology
Elevated plus maze
Excitatory Amino Acid Agents - isolation & purification
Excitatory Amino Acid Agents - pharmacology
Fatty Acids - isolation & purification
Fatty Acids - pharmacology
GABA Agents - isolation & purification
GABA Agents - pharmacology
Male
Maze Learning - drug effects
Maze Learning - physiology
Mice
Mice, Inbred ICR
Nervousness
Plant Extracts - isolation & purification
Plant Extracts - pharmacology
Plant Leaves
Plant Stems
Serotonin Agents - isolation & purification
Serotonin Agents - pharmacology
Terpenes - isolation & purification
Terpenes - pharmacology
Verbenaceae
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Summary:Aloysia triphylla (Verbenaceae) is an aromatic medicinal plant, and it is used for the treatment of “nervous” problems as, “sadness” and “nervousness”. While, there are no reports about its pharmacological activity in animal models. The objective of this work was to evaluate the anxiolytic effect of the extracts and fractions of this species and to measure the interaction of the most active fraction with serotonergic, glutamatergic and GABAergic drugs. An elevated plus maze test was carried ought where the methanol (AtM), dicloromethane (AtD) and hexanic (AtH) extracts presented anxiolytic activity in mice when exposed to the test. Also, different fractions obtained from the AtD were evaluated (AtF1, AtF2 and AtF3, 15mg/kg), and showed that fraction AtF1 possessed the anxiolytic activity, in the same model. Then, AtF1 was co-administered with different drugs, which act on GABAergic (bicuculline, picrotoxin, pentylenetetrazol, baclofen and phaclofen), or serotononinergic (DOI, 8-OH-DPAT, WAY 100635 and ketanserine) or glutamatergic (NMDA, MPEP and MK-801) systems. The anxiolytic activity of AtF1 was modified by GABAergic and serotoninergic drugs. Chemical analysis of this fraction by using GC–MS, showed that it contains hexadecanoic acid, hexadecanoic acid methyl ester, octadecanoic acid methyl ester, eicosanoic acid methyl ester, vitamin E, α-amiryn, campesterol, sitosterol, stigmastan-2,22, dien-3-ol (4) and stigmasta 5, 24 (28) dien-3-ol.
ISSN:0753-3322
1950-6007
DOI:10.1016/j.biopha.2017.10.024