Selective Targeting of Vibrios by Fluorescent Siderophore-Based Probes

Siderophores are small molecules used to specifically transport iron into bacteria via related receptors. By adapting siderophores and hijacking their pathways, we may discover an efficient and selective way to target microbes. Herein, we report the synthesis of a siderophore-fluorophore conjugate V...

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Bibliographic Details
Published in:ACS chemical biology 2017-11, Vol.12 (11), p.2720-2724
Main Authors: Chen, Peng-Hsun Chase, Ho, Sheng-Yang, Chen, Pin-Lung, Hung, Tzu-Chiao, Liang, An-Jou, Kuo, Tang-Feng, Huang, Hsiao-Chun, Wang, Tsung-Shing Andrew
Format: Article
Language:English
Subjects:
Biological Transport
Citrates - chemical synthesis
Citrates - chemistry
Citrates - metabolism
Escherichia coli - metabolism
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
Fluorescent Dyes - metabolism
Humans
Microscopy, Fluorescence - methods
Pyrrolidinones - chemical synthesis
Pyrrolidinones - chemistry
Pyrrolidinones - metabolism
Siderophores - chemical synthesis
Siderophores - chemistry
Siderophores - metabolism
Staphylococcus aureus - metabolism
Vibrio - isolation & purification
Vibrio - metabolism
Vibrio Infections - microbiology
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Summary:Siderophores are small molecules used to specifically transport iron into bacteria via related receptors. By adapting siderophores and hijacking their pathways, we may discover an efficient and selective way to target microbes. Herein, we report the synthesis of a siderophore-fluorophore conjugate VF-FL derived from vibrioferrin (VF). Using flow cytometry and fluorescence microscopy, the probe selectively labeled vibrios, including V. parahaemolyticus, V. cholerae, and V. vulnificus, even in the presence of other species such as S. aureus and E. coli. The labeling is siderophore-related and both iron-limited conditions and the siderophore moiety are required. The competitive relationship between VF-FL and VF in vibrios implies an unreported VF-related transport mechanism in V. cholerae and V. vulnificus. These studies demonstrate that the siderophore scaffold provides a method to selectively target microbes expressing cognate receptors under iron-limited conditions.
ISSN:1554-8929
1554-8937
DOI:10.1021/acschembio.7b00667