Phosphoglycerate kinase acts as a futile cycle at high temperature

In (hyper)thermophilic organisms metabolic processes have to be adapted to function optimally at high temperature. We compared the gluconeogenic conversion of 3-phosphoglycerate via 1,3-bisphosphoglycerate to glyceraldehyde-3-phosphate at 30 °C and at 70 °C. At 30 °C it was possible to produce 1,3-b...

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Bibliographic Details
Published in:Microbiology (Society for General Microbiology) 2017-11, Vol.163 (11), p.1604-1612
Main Authors: Kouril, Theresa, Eicher, Johann J, Siebers, Bettina, Snoep, Jacky L
Format: Article
Language:English
Subjects:
Enzyme Stability
Gluconeogenesis
Glyceraldehyde 3-Phosphate - metabolism
Glyceraldehyde-3-Phosphate Dehydrogenases - chemistry
Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism
Glyceric Acids - metabolism
Half-Life
Hot Temperature
Kinetics
Models, Statistical
Phosphoglycerate Kinase - chemistry
Phosphoglycerate Kinase - metabolism
Recombinant Proteins - chemistry
Recombinant Proteins - metabolism
Saccharomyces cerevisiae - enzymology
Substrate Cycling - physiology
Sulfolobus solfataricus - enzymology
Thermodynamics
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Summary:In (hyper)thermophilic organisms metabolic processes have to be adapted to function optimally at high temperature. We compared the gluconeogenic conversion of 3-phosphoglycerate via 1,3-bisphosphoglycerate to glyceraldehyde-3-phosphate at 30 °C and at 70 °C. At 30 °C it was possible to produce 1,3-bisphosphoglycerate from 3-phosphoglycerate with phosphoglycerate kinase, but at 70 °C, 1,3-bisphosphoglycerate was dephosphorylated rapidly to 3-phosphoglycerate, effectively turning the phosphoglycerate kinase into a futile cycle. When phosphoglycerate kinase was incubated together with glyceraldehyde 3-phosphate dehydrogenase it was possible to convert 3-phosphoglycerate to glyceraldehyde 3-phosphate, both at 30 °C and at 70 °C, however, at 70 °C only low concentrations of product were observed due to thermal instability of glyceraldehyde 3-phosphate. Thus, thermolabile intermediates challenge central metabolic reactions and require special adaptation strategies for life at high temperature.
ISSN:1350-0872
1465-2080
DOI:10.1099/mic.0.000542