Novel screening strategy reveals a potent Bacillus antagonist capable of mitigating wheat take‐all disease caused by Gaeumannomyces graminis var. tritici

Take‐all is a severe root disease of wheat worldwide that is caused by the soilborne fungal pathogen Gaeumannomyces graminis var. tritici (Ggt). In this study, 272 Bacillus isolates were screened for their antifungal activity in vitro to Ggt. Of the 128 strains that demonstrated an antagonistic acti...

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Bibliographic Details
Published in:Letters in applied microbiology 2017-12, Vol.65 (6), p.512-519
Main Authors: Zhang, D.D., Guo, X.J., Wang, Y.J., Gao, T.G., Zhu, B.C.
Format: Article
Language:English
Subjects:
Acetic acid
Antibiotics
Antifungal activity
Antifungal Agents - metabolism
Ascomycota - growth & development
Bacillus
Bacillus - genetics
Bacillus - isolation & purification
Bacillus - metabolism
Bacillus antagonist
biocontrol
Biological control
Biological Control Agents - metabolism
Disease control
Fungi
Fungicides
Gaeumannomyces graminis tritici
Gaeumannomyces graminis var. tritici
Indoleacetic acid
lipopeptide
Lipopeptides
MALDI‐TOF‐MS
Organic phosphorus
Pathogens
Phosphorus
Plant diseases
Plant Diseases - microbiology
Plant growth
Plant Roots - microbiology
Plants (botany)
Promotion
Screening
screening strategy
Solubilization
Surfactin
Take-all disease
Triticum - microbiology
Wheat
wheat take‐all
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Summary:Take‐all is a severe root disease of wheat worldwide that is caused by the soilborne fungal pathogen Gaeumannomyces graminis var. tritici (Ggt). In this study, 272 Bacillus isolates were screened for their antifungal activity in vitro to Ggt. Of the 128 strains that demonstrated an antagonistic action, 24 of these exhibited at least three of the four plant growth promotion parameters (i.e. indole acetic acid and siderophore production, inorganic phosphorus solubilization and organic phosphorus solubilization) that were tested in wheat plants. The most effective strain found was Bacillus subtilis Pnf‐12; its disease reduction effect reached 69%. Pnf‐12 also caused a significant improvement (P  0·05). The mechanism for this disease control may be linked to the production of the antifungal lipopeptides surfactin, iturin and fengycin production, all of which were detected in the cell‐free supernatant of Pnf‐12. Significance and Impact of the Study Take‐all, which is caused by the soilborne fungal pathogen Gaeumannomyces graminis var. tritici (Ggt), is one of the most widespread and devastating root diseases of wheat plants. This study focuses on a novel screening strategy of Bacillus isolates to evaluate their potential biological control capacity for suppressing wheat take‐all. The joint assessment of antifungal activities, growth promotion factors and variety of antibiotic synthesis genes, in addition to greenhouse experiments, allowed for the identification and demonstration of the Bacillus isolate Pnf‐12 as an effective disease control agent. Significance and Impact of the Study: Take‐all, which is caused by the soilborne fungal pathogen Gaeumannomyces graminis var. tritici (Ggt), is one of the most widespread and devastating root diseases of wheat plants. This study focuses on a novel screening strategy of Bacillus isolates to evaluate their potential biological control capacity for suppressing wheat take‐all. The joint assessment of antifungal activities, growth promotion factors and variety of antibiotic synthesis genes, in addition to greenhouse experiments, allowed for the identification and demonstration of the Bacillus isolate Pnf‐12 as an effective disease control agent.
ISSN:0266-8254
1472-765X
DOI:10.1111/lam.12809