Simplified quantification of small animal [18F]FDG PET studies using a standard arterial input function

Arterial input function (AIF) measurement for quantification of small animal PET studies is technically challenging and limited by the small blood volume of small laboratory animals. The present study investigated the use of a standard arterial input function (SAIF) to simplify the experimental proc...

Full description

Saved in:
Bibliographic Details
Published in:European journal of nuclear medicine and molecular imaging 2006-08, Vol.33 (8), p.948-954
Main Authors: Meyer, Philipp T, Circiumaru, Valentina, Cardi, Christopher A, Thomas, Daniel H, Bal, Harshali, Acton, Paul D
Format: Article
Language:English
Subjects:
Algorithms
Animals
Arteries - diagnostic imaging
Arteries - metabolism
Brain
Computer Simulation
Fluorodeoxyglucose F18 - blood
Fluorodeoxyglucose F18 - pharmacokinetics
Image Interpretation, Computer-Assisted - methods
Male
Medical imaging
Models, Cardiovascular
Positron-Emission Tomography - methods
Positron-Emission Tomography - veterinary
Radioisotope Dilution Technique
Radiopharmaceuticals - blood
Radiopharmaceuticals - pharmacokinetics
Rats
Rats, Sprague-Dawley
Rodents
Validation studies
Veins & arteries
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Arterial input function (AIF) measurement for quantification of small animal PET studies is technically challenging and limited by the small blood volume of small laboratory animals. The present study investigated the use of a standard arterial input function (SAIF) to simplify the experimental procedure. Twelve [(18)F]fluorodeoxyglucose ([(18)F]FDG) PET studies accompanied by serial arterial blood sampling were acquired in seven male Sprague-Dawley rats under isoflurane anaesthesia without (every rat) and with additional (five rats) vibrissae stimulation. A leave-one-out procedure was employed to validate the use of a SAIF with individual scaling by one (1S) or two (2S) arterial blood samples. Automatic slow bolus infusion of [(18)F]FDG resulted in highly similar AIF in all rats. The average differences of the area under the curve of the measured AIF and the individually scaled SAIF were 0.11+/-4.26% and 0.04+/-2.61% for the 1S (6-min sample) and the 2S (4-min/43-min samples) approach, respectively. The average differences between the cerebral metabolic rates of glucose (CMR(glc)) calculated using the measured AIF and the scaled SAIF were 1.31+/-5.45% and 1.30+/-3.84% for the 1S and the 2S approach, respectively. The use of a SAIF scaled by one or (preferably) two arterial blood samples can serve as a valid substitute for individual AIF measurements to quantify [(18)F]FDG PET studies in rats. The SAIF approach minimises the loss of blood and should be ideally suited for longitudinal quantitative small animal [(18)F]FDG PET studies.
ISSN:1619-7070
1619-7089
DOI:10.1007/s00259-006-0121-7