Orabase formulation with cashew gum polysaccharide decreases inflammatory and bone loss hallmarks in experimental periodontitis

This study aimed to investigate the chemical characteristics and the effects of an orabase gel with Cashew Gum Polysaccharide (CG-P) from Anacardium occidentale L. on alveolar bone loss and relative mRNA expression of TNF-α, IL-1β, RANK, RANKL, and OPG in the periodontal tissue of Wistar rats (Rattu...

Full description

Saved in:
Bibliographic Details
Published in:International journal of biological macromolecules 2018-02, Vol.107 (Pt A), p.1093-1101
Main Authors: Souza Filho, Manoel D., Medeiros, Jand V.R., Vasconcelos, Daniel F.P., Silva, Durcilene A., Leódido, Ana C.M., Fernandes, Hygor F., Silva, Felipe R.P., França, Luiz F.C., Lenardo, David, Pinto, Giovanny R.
Format: Article
Language:English
Subjects:
Alveolar Bone Loss - drug therapy
Alveolar Bone Loss - genetics
Alveolar Bone Loss - pathology
Anacardium - chemistry
Anacardium occidentale
Animals
Anti-inflammatory
Carboxymethylcellulose Sodium - administration & dosage
Carboxymethylcellulose Sodium - analogs & derivatives
Carboxymethylcellulose Sodium - chemistry
Disease Models, Animal
Female
Gene expression
Gene Expression Regulation - drug effects
Humans
Inflammation - drug therapy
Inflammation - genetics
Inflammation - pathology
Interleukin-1beta - genetics
Oxidative Stress - drug effects
Periodontitis - drug therapy
Periodontitis - genetics
Periodontitis - pathology
Peroxidase - genetics
Polysaccharides - administration & dosage
Polysaccharides - chemistry
RANK Ligand - genetics
Rats
Rats, Wistar
Tumor Necrosis Factor-alpha - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study aimed to investigate the chemical characteristics and the effects of an orabase gel with Cashew Gum Polysaccharide (CG-P) from Anacardium occidentale L. on alveolar bone loss and relative mRNA expression of TNF-α, IL-1β, RANK, RANKL, and OPG in the periodontal tissue of Wistar rats (Rattus norvegicus) subjected to ligature-induced periodontitis. Crude cashew gum was collected and purified by chemical processes; then, the CG-P was mixed with orabase gel. Female rats were randomly divided into four groups of six animals each: saline 0.9% (Sal Group); orabase gel (Gel Group); 50mg CG-P/1g orabase gel (CG-P50 Group) and 150mg CG-P/1g orabase gel (CG-P150 Group). Periodontitis was induced in the animals; they were treated for 20days with one daily topical application. The purification process of CG-P presented high yield and resulted in a protein-free product. The treatment with CG-P150 (150mg CG-P/1g orabase gel) significantly reduced alveolar bone loss, decreased the relative mRNA expression of TNF-α, IL-1β, RANKL and the RANKL/OPG ratio, and caused a significant decrease in myeloperoxidase activity of the gingival tissue. Thus, the CG-P in orabase represents a potential adjuvant drug for the treatment of periodontitis and possible source of new biotechnological discoveries.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2017.09.087