Intracellular translocation of glutathione S-transferase pi during oligodendrocyte differentiation in adult rat cerebral cortex in vivo

Abstract Glutathione S-transferase (GST)-pi is a cytosolic isoenzyme used as a marker for mature oligodendrocytes in the mammalian brain. However, the cellular properties of GST-pi-immunoreactive [GST-pi (+)] cells in adult brain are not completely understood. We immunohistochemically demonstrated t...

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Bibliographic Details
Published in:Neuroscience 2007-08, Vol.148 (2), p.535-540
Main Authors: Tamura, Y, Kataoka, Y, Cui, Y, Takamori, Y, Watanabe, Y, Yamada, H
Format: Article
Language:English
Subjects:
2',3'-Cyclic-Nucleotide Phosphodiesterases - metabolism
Animals
Antigens - metabolism
Biological and medical sciences
BrdU
Bromodeoxyuridine - metabolism
Carbocyanines - metabolism
Cell Count - methods
Cell Differentiation - physiology
Cerebral Cortex - cytology
CNPase
Fundamental and applied biological sciences. Psychology
Glutathione S-Transferase pi - metabolism
Lamin Type B - metabolism
Male
Neurology
NG2
oligodendrocyte progenitor cells
Oligodendroglia - classification
Oligodendroglia - enzymology
Protein Transport - physiology
Proteoglycans - metabolism
Rats
Rats, Wistar
RIP
Vertebrates: nervous system and sense organs
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Summary:Abstract Glutathione S-transferase (GST)-pi is a cytosolic isoenzyme used as a marker for mature oligodendrocytes in the mammalian brain. However, the cellular properties of GST-pi-immunoreactive [GST-pi (+)] cells in adult brain are not completely understood. We immunohistochemically demonstrated the existence of two subtypes of GST-pi (+) cells in the cerebral cortex of adult rats: one subtype exhibited GST-pi in the cytoplasm (C-type cells), while the other did mainly in the nucleus (N-type cells). The GST-pi (+) C-type cells were also immunopositive for 2′,3′-cyclic nucleotide 3′-phosphodiesterase and RIP, indicating that they were mature oligodendrocytes, while the GST-pi (+) N-type cells expressed NG2, indicating that they were oligodendrocyte progenitor cells. Furthermore, observation of the fate of newly-generated cells by 5-bromodeoxyuridine-labeling revealed that the GST-pi (+) N-type cells differentiated into C-type cells. These findings indicate translocation of GST-pi from the nucleus to the cytoplasm during oligodendrocyte maturation.
ISSN:0306-4522
1873-7544
DOI:10.1016/j.neuroscience.2007.06.026