S. Typhimurium virulence changes caused by exposure to different non-thermal preservation treatments using C. elegans

The aims of this research study were: (i) to postulate Caenorhabditis elegans (C. elegans) as a useful organism to describe infection by Salmonella enterica serovar Typhimurium (S. Typhimurium), and (ii) to evaluate changes in virulence of S. Typhimurium when subjected repetitively to different anti...

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Bibliographic Details
Published in:	International journal of food microbiology 2017-12, Vol.262, p.49-54
Main Authors:	Sanz-Puig, María, Lázaro, Elena, Armero, Carmen, Alvares, Danilo, Martínez, Antonio, Rodrigo, Dolores
Format:	Article
Language:	eng
Subjects:	Animals Anti-Bacterial Agents - pharmacology Antiinfectives and antibacterials Antimicrobial Antimicrobial agents Bayes Theorem Bayesian analysis Bayesian survival analysis Brassica - metabolism Byproducts Caenorhabditis elegans - microbiology Disease Models, Animal E coli Electric fields Escherichia coli - pathogenicity Foodborne Diseases - microbiology High Hydrostatic Pressure Hydrostatic Pressure Infections Life span Microorganisms Plant Preparations - pharmacology Populations Preservation Pulsed Electric Fields Salmonella Salmonella Infections - microbiology Salmonella Infections - pathology Salmonella typhimurium - pathogenicity Survival analysis Virulence
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Summary:	The aims of this research study were: (i) to postulate Caenorhabditis elegans (C. elegans) as a useful organism to describe infection by Salmonella enterica serovar Typhimurium (S. Typhimurium), and (ii) to evaluate changes in virulence of S. Typhimurium when subjected repetitively to different antimicrobial treatments. Specifically, cauliflower by-product infusion, High Hydrostatic Pressure (HHP), and Pulsed Electric Fields (PEF). This study was carried out by feeding C. elegans with different microbial populations: E. coli OP50 (optimal conditions), untreated S. Typhimurium, S. Typhimurium treated once and three times with cauliflower by-product infusion, S. Typhimurium treated once and four times with HHP and S. Typhimurium treated once and four times with PEF. Bayesian survival analysis was applied to estimate C. elegans lifespan when fed with the different microbial populations considered. Results showed that C. elegans is a useful organism to describe infection by S. Typhimurium because its lifespan was reduced when it was infected. In addition, the application of antimicrobial treatments repetitively generated different responses: when cauliflower by-product infusion and PEF treatment were applied repetitively the virulence of S. Typhimurium was lower than when the treatment was applied once. In contrast, when HHP treatment was applied repetitively, the virulence of S. Typhimurium was higher than when it was applied once. Nevertheless, in all the populations analyzed treated S. Typhimurium had lower virulence than untreated S. Typhimurium. •S. Typhimurium became resistant to antimicrobial infusion, HHP and PEF.•C. elegans was postulated as a useful organism to describe S. Typhimurium infection.•Except for high pressure, repeated antimicrobial exposure decreased S. Typhimurium virulence.
ISSN:	0168-1605 1879-3460