A monoclonal antibody-based enzyme-linked immunosorbent assay for the determination of chlorogenic acid in honeysuckle

[Display omitted] •A highly specific monoclonal antibody against Chlorogenic acid (CGA) was produced.•An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed based on the monoclonal antibody.•The proposed icELISA is a reliable, simple and cost effective system for the rapid...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2018-01, Vol.148, p.1-5
Main Authors: Zhang, Bo, Nan, Tie-Gui, Zhan, Zhi-Lai, Kang, Li-Ping, Yang, Jian, Lai, Chang-Jiang-Sheng, Yuan, Yuan, Wang, Bao-Min, Huang, Lu-Qi
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - chemistry
China
Chlorogenic acid
Chlorogenic Acid - chemistry
Chromatography, High Pressure Liquid - methods
Cross Reactions
ELISA
Enzyme-Linked Immunosorbent Assay - methods
Female
Flowers - chemistry
Honeysuckle
Lonicera - chemistry
Mice
Mice, Inbred BALB C
Monoclonal antibody
Plants, Medicinal - chemistry
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Summary:[Display omitted] •A highly specific monoclonal antibody against Chlorogenic acid (CGA) was produced.•An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed based on the monoclonal antibody.•The proposed icELISA is a reliable, simple and cost effective system for the rapid detection of CGA in honeysuckle. The edible flower buds of honeysuckle are traditionally used as herbal medicine or food additives in China. Chlorogenic acid (CGA) serves as the quality control marker of honeysuckle for its high content and antipyretic property. In this paper, a specific monoclonal antibody (mAb2E2) against CGA was produced. After optimization, mAb2E2-based indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed. The concentrations of CGA producing 50% inhibition and the calibration range of the icELISA were 0.39 and 0.10–1.51ng/mL, respectively. The icELISA had cross-reactivity with 3,5-Dicaffeoylquinic acid (17.53%), and the cross-reactivity levels with other analogs were all below 5%. The average recoveries obtained by standard CGA addition to honeysuckle samples were from 88.4 to 104.8%. The icELISA was applied to CGA detection in different honeysuckle samples and the results were confirmed by high-performance liquid chromatography analysis. The correlation coefficient between the two assays was 0.97. The proposed icELISA provides a feasible analytical method for highly sensitive and specific, simple, fast, and high-throughput determination of CGA in honeysuckle samples.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2017.09.023