Exploitation of agro-climatic environment for selection of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase producing salt tolerant indigenous plant growth promoting rhizobacteria

A comprehensive survey for 09 agro-climatic zones of Uttar Pradesh, India was conducted to isolate and characterize salt tolerant 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase possessing plant growth promoting (PGP) rhizobacteria for salt stress amelioration in rice. Here, we have isolated 1...

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Bibliographic Details
Published in:Microbiological research 2017-12, Vol.205, p.25-34
Main Authors: Misra, Sankalp, Dixit, Vijay Kant, Khan, Mohammad Haneef, Kumar Mishra, Shashank, Dviwedi, Gyanendra, Yadav, Sumit, Lehri, Alok, Singh Chauhan, Puneet
Format: Article
Language:English
Subjects:
16S rRNA
Abiotic stress
ACC deaminase
Agroclimatic zones
Bacteria - classification
Bacteria - enzymology
Bacteria - genetics
Bacteria - isolation & purification
Biofilms - growth & development
Biomass
Carbon-Carbon Lyases - metabolism
DNA, Bacterial - genetics
Environment
Geographic Mapping
Germination
Hydrogen-Ion Concentration
India
Indigenous
Indoleacetic Acids - metabolism
Oryza - microbiology
Oryza - physiology
PGPR
Phosphates - chemistry
Phylogeny
Plant Development
Plant Roots - microbiology
RNA, Ribosomal, 16S - genetics
Salinity
Salt-Tolerance - physiology
Salt-Tolerant Plants - growth & development
Salt-Tolerant Plants - microbiology
Seedlings - growth & development
Seedlings - microbiology
Sequence Analysis
Siderophores - metabolism
Soil - chemistry
Soil Microbiology
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Summary:A comprehensive survey for 09 agro-climatic zones of Uttar Pradesh, India was conducted to isolate and characterize salt tolerant 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase possessing plant growth promoting (PGP) rhizobacteria for salt stress amelioration in rice. Here, we have isolated 1125 bacteria having the ability to tolerate 1M NaCl and out of those, 560 were screened for utilizing ACC as sole nitrogen source. 560 isolates were subjected for bacteria coated seed germination assay under 100mM salt (NaCl) stress resulting to 77 isolates which were further evaluated for seed germination assay, PGP and abiotic stress tolerance ability in vitro. This evaluation revealed 15 potent rhizobacteria representing each agro-climatic zone and salt stress mitigation in vitro. In particular, the biomass obtained for bacteria coated rice seedlings were corroborated with the performance of isolates exhibiting maximum average indole acetic acid (IAA) production respective to the agro-climatic zone. Surprisingly based on 16S rRNA, much of the propitious isolates belonged to same specific epithet exhibited variedly in their characteristics. Overall, Bacillus spp. was explored as dominant genera in toto with highest distribution in Western Plain zone followed by Central zone. Therefore, this study provides a counter-intuitive perspective of selection of native microflora for their multifarious PGP and abiotic stress tolerance abilities based on the agro-climatic zones to empower the establishment and development of more suitable inoculants for their application in agriculture under local stress environments.
ISSN:0944-5013
1618-0623
DOI:10.1016/j.micres.2017.08.007