Does l-glutamine-supplemented diet extenuate NO-mediated damage on myenteric plexus of Walker 256 tumor-bearing rats?

This study was designed to appraise the relationship between enteric neuropathy and oxidative stress in cancer cachexia under l-glutamine-supplemented diet. Total and nitrergic neuronal populations were investigated in jejunum and ileum in four experimental groups: control (C); control l-glutamine-s...

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Bibliographic Details
Published in:Food research international 2017-11, Vol.101, p.24-34
Main Authors: Vicentini, Geraldo Emílio, Martins, Heber Amilcar, Fracaro, Luciane, de Souza, Sara Raquel Garcia, da Silva Zanoni, Kassio Papi, Silva, Thamara Nishida Xavier, Blegniski, Fernanda Paschoal, Guarnier, Flávia Alessandra, Zanoni, Jacqueline Nelisis
Format: Article
Language:English
Subjects:
Animals
Antioxidants
Cachexia
Cachexia - diet therapy
Cachexia - metabolism
Cachexia - pathology
Carcinoma 256, Walker - diet therapy
Carcinoma 256, Walker - pathology
Dietary Supplements
Disease Models, Animal
Enteric neurons
Glutamine - administration & dosage
Glutamine - pharmacology
Glutamine - therapeutic use
Ileum - drug effects
Ileum - metabolism
Ileum - pathology
Jejunum - drug effects
Jejunum - metabolism
Jejunum - pathology
l-glutamine
Male
Myenteric Plexus - drug effects
Neurons
Nitric oxide
Nitric Oxide - adverse effects
Nitric Oxide Synthase Type I - metabolism
Oxidative Stress - drug effects
Rats
Rats, Wistar
tert-Butylhydroperoxide - adverse effects
Tumor Burden
Walker 256 tumor
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Summary:This study was designed to appraise the relationship between enteric neuropathy and oxidative stress in cancer cachexia under l-glutamine-supplemented diet. Total and nitrergic neuronal populations were investigated in jejunum and ileum in four experimental groups: control (C); control l-glutamine-supplemented diet (CG); Walker-256 tumor (TW); and Walker-256 tumor supplemented with l-glutamine (TWG). In addition, local oxidative stress, neuronal nitric oxide synthase (nNOS) enzyme and nitric oxide (NO) levels were evaluated. Neuronal density and somatic area of the total and nitrergic populations were reduced in TW rats, which was accompanied by high oxidative stress, NO and nNOS levels. l-glutamine supplementation prevented neuronal atrophy, changes in pan neuronal density and nNOS overexpression (ileum), and restored total antioxidant capacity. Nevertheless, the oxidative stress was partially mitigated and no effect was observed on the reduction of nitrergic population and NO levels. l-glutamine-supplemented diet extenuates NO-mediated damage on the myenteric plexus although has a small benefit on oxidative stress. [Display omitted] •Enteric innervation damage was demonstrated in cancer cachexia rat model.•Cachectic rats showed high lipid peroxidation and NO levels in jejunum samples.•Enteric neuropathy was partially prevented by l-glutamine-supplemented diet.•The higher nNOS expression was prevented by l-glutamine in the ileum exclusively.
ISSN:0963-9969
1873-7145
DOI:10.1016/j.foodres.2017.08.054