Assessment of the Intestinal Barrier with Five Different Permeability Tests in Healthy C57BL/6J and BALB/cJ Mice

Background Intestinal permeability is thought to be of major relevance for digestive and nutrition-related diseases, and therefore has been studied in numerous mouse models of disease. However, it is unclear which tools are the preferable ones, and how normal values should be defined. Aims To compar...

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Bibliographic Details
Published in:Digestive diseases and sciences 2016-03, Vol.61 (3), p.737-746
Main Authors: Volynets, Valentina, Reichold, Astrid, Bárdos, Gyöngyi, Rings, Andreas, Bleich, André, Bischoff, Stephan C.
Format: Article
Language:English
Subjects:
Albumin
Analysis
Animals
Biochemistry
Dextran
Dextrans - blood
Dextrans - metabolism
Female
Fluorescein-5-isothiocyanate - analogs & derivatives
Fluorescein-5-isothiocyanate - metabolism
Gastroenterology
Hepatology
Intestinal Mucosa - metabolism
Lactulose - metabolism
Lactulose - urine
Lipopolysaccharides - blood
Lipopolysaccharides - metabolism
Male
Mannitol - metabolism
Mannitol - urine
Medical research
Medicine
Medicine & Public Health
Medicine, Experimental
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Oncology
Original Article
Ovalbumin - blood
Ovalbumin - metabolism
Permeability
Polyethylene glycol
Polyethylene Glycols - metabolism
Polyols
Portal Vein
Transplant Surgery
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Summary:Background Intestinal permeability is thought to be of major relevance for digestive and nutrition-related diseases, and therefore has been studied in numerous mouse models of disease. However, it is unclear which tools are the preferable ones, and how normal values should be defined. Aims To compare different in vivo permeability tests in healthy mice of commonly used genetic backgrounds. Methods We assessed the intestinal barrier in male and female C57BL/6J and BALB/cJ mice of different ages, using four orally administered permeability markers, FITC-dextran 4000 (FITC-D4000) and ovalbumin (OVA) measured in plasma, and polyethylene glycol (PEG) and lactulose/mannitol (Lac/Man) measured in urine, and by assessing lipopolysaccharide (LPS) in portal vein plasma. Results After gavage, FITC-D4000, OVA, Lac/Man, and PEG400, but not PEG4000, were detectable in plasma or urine. Female mice tended to have a higher permeability according to the FITC-D4000, OVA, and PEG400 tests, but the Lac/Man ratio was higher in males. No significant differences between the two mouse strains of young and old mice were observed except for mannitol recovery, which was higher in BALB/cJ mice compared to C57BL/6J mice ( p  
ISSN:0163-2116
1573-2568
DOI:10.1007/s10620-015-3935-y