Extraction, purification, and characterization of the polysaccharides from Opuntia milpa alta

Polysaccharides production from Opuntia milpa alta was carried out. The effects of the extraction parameters of extraction time, extraction temperature and ratio of water to raw material were optimized by using statistical techniques such as a three factor, three level Box–Behnken factorial design (...

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Bibliographic Details
Published in:Carbohydrate polymers 2008-02, Vol.71 (3), p.403-410
Main Authors: Cai, Weirong, Gu, Xiaohong, Tang, Jian
Format: Article
Language:English
Subjects:
Applied sciences
Biological and medical sciences
Chemical constitution
duration
equations
Exact sciences and technology
Extraction
Fundamental and applied biological sciences. Psychology
medicinal plants
Natural polymers
Optimization
Opuntia
Opuntia milpa alta
Physicochemistry of polymers
plant extracts
Plant physiology and development
Polysaccharides
Purification
Response surface analysis
Starch and polysaccharides
statistical analysis
temperature
water
yields
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Summary:Polysaccharides production from Opuntia milpa alta was carried out. The effects of the extraction parameters of extraction time, extraction temperature and ratio of water to raw material were optimized by using statistical techniques such as a three factor, three level Box–Behnken factorial design (BBD) and response surface methodology (RSM), with a view to maximize the extraction yield under optimal extraction parameters combination. From this study, it could be concluded that the maximum yield of the polysaccharides (0.694%) can be obtained extraction temperature of 86.1 °C, extraction time of 3.61 h, ratio of water to material of 3.72:1. Three isolated fractions of the polysaccharides were characterized by employing High-Performance Liquid Chromatography (HPLC).
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2007.06.008