Stem cells and differentiated cells differ in their sensitivity to urine in vitro

Urinary tract regeneration using tissue engineering is one of the most challenging issues in the field of reconstructive urology. Cells seeded on scaffold are exposed to urine immediately after the implantation. The outcome of urinary bladder regeneration is depended on the ability of these cells to...

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Bibliographic Details
Published in:Journal of cellular biochemistry 2018-02, Vol.119 (2), p.2307-2319
Main Authors: Pokrywczyńska, Marta, Kloskowski, Tomasz, Balcerczyk, Daria, Buhl, Monika, Jundziłł, Arkadiusz, Nowacki, Maciej, Męcińska‐Jundziłł, Kaja, Drewa, Tomasz
Format: Article
Language:English
Subjects:
Adipose tissue
Adipose Tissue - cytology
Adipose Tissue - metabolism
Animals
Apoptosis
Biotechnology
Bladder
Bladder cancer
Cancer
Cell Differentiation
Cell Proliferation
Cell Survival
Cells, Cultured
Cytometry
Cytotoxicity
Flow cytometry
Humans
Implantation
In Vitro Techniques
Incubation
Mesenchyme
Muscles
Regeneration
Sensitivity
Smooth muscle
Stem cell transplantation
Stem cells
Stem Cells - cytology
Stem Cells - metabolism
Swine
Tissue Engineering
Toxicity
Urinary bladder
Urinary Bladder - cytology
Urinary Bladder - metabolism
Urinary tract
Urine
Urine - chemistry
Urology
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Summary:Urinary tract regeneration using tissue engineering is one of the most challenging issues in the field of reconstructive urology. Cells seeded on scaffold are exposed to urine immediately after the implantation. The outcome of urinary bladder regeneration is depended on the ability of these cells to survive, proliferate, and regenerate. The aim of this study was to compare a sensitivity of three different cell lines to urine in vitro. Three different cell lines were isolated from porcine bladder (urothelial cells, UCs and smooth muscle cells, SMCs) and adipose tissue (adipose‐derived stem cells, ADSCs). Cell viability (MTT assay), proliferation (real‐time cell analysis using xCELLigence system) and apoptosis/necrosis (flow cytometry) were analyzed after exposition to urine. ADSCs were the most sensitive to urine compared to two other tested cell lines. Among the bladder cell lines the UCs were more resistant to urine than SMCs. Twenty four hour incubation of UCs, SMCs, and ADSCs with urine lead to ∼40%, ∼70%, and ∼90% reduction of their viability, respectively. The mechanism of urine mediated cytotoxicity differed depending on the tested cell type. Urothelial and SMCs seems to be more suitable for urinary bladder regeneration compared to mesenchymal stem cells, however, these cells have limited application especially in the case of urinary bladder cancer. In this study, we compared the sensitivity of three different cell lines to urine environment in vitro. Differentiated cells isolated from bladder biopsy seems to be more suitable for regeneration of urinary tract, however, these cells have limited application especially in the case of urinary bladder cancer. ADSCs were the most sensitive on urine among all tested cell lines, in order to reduce sensitivity of MSCs on urine, initial predifferentiation could be performed before implantation which could increase their viability.
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.26393