Evaluation of the effect of four final irrigation protocols on root canal dentin components by polarized light microscopy and scanning electron microscopy

The present study aimed to evaluate the effects of four endodontic chelating agents, followed by 2.5% sodium hypochlorite (NaOCl), as final irrigation regimens on organic and inorganic components of human root dentin. Sixty mandibular incisors were prepared and randomly divided into six groups (n = ...

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Bibliographic Details
Published in:Microscopy research and technique 2017-12, Vol.80 (12), p.1337-1343
Main Authors: Baldasso, Flávia Emi Razera, Cardoso, Luana Roleto, Silva, Vinicius Duval da, Morgental, Renata Dornelles, Kopper, Patrícia Maria Poli
Format: Article
Language:English
Subjects:
Chelating agents
Chelation
Citric acid
Collagen
Dentin
Distilled water
Edetic acid
Electron microscopy
Endodontics
Ethylenediaminetetraacetic acids
Incisors
Irrigation water
Lavage
Light microscopy
Mandible
Peracetic acid
Polarized light
Protocol (computers)
root canal irrigation
Root canals
Scanning electron microscopy
Smear
Sodium
Sodium hypochlorite
Structural analysis
Tubules
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Summary:The present study aimed to evaluate the effects of four endodontic chelating agents, followed by 2.5% sodium hypochlorite (NaOCl), as final irrigation regimens on organic and inorganic components of human root dentin. Sixty mandibular incisors were prepared and randomly divided into six groups (n = 10): QMiX, 1% peracetic acid (PA), 17% EDTA, 10% citric acid (CA), 2.5% NaOCl (solution control) and distilled water (DW‐negative control). After irrigation with the chelating agents, a final flush was performed with 2.5% NaOCl. The specimens were split longitudinally in halves; one was designated for organic component analysis by polarized light microscopy (PLM) and the other for inorganic structure analysis by scanning electron microscopy (SEM). Scores data obtained in the PLM analysis were submitted to Kruskal‐Wallis' test, followed by Dunn's test (p 
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.22946