A Synthetic Glycan Microarray Enables Epitope Mapping of Plant Cell Wall Glycan-Directed Antibodies

In the last three decades, more than 200 monoclonal antibodies have been raised against most classes of plant cell wall polysaccharides by different laboratories worldwide. These antibodies are widely used to identify differences in plant cell wall components in mutants, organ and tissue types, and...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 2017-11, Vol.175 (3), p.1094-1104
Main Authors: Ruprecht, Colin, Bartetzko, Max P., Senf, Deborah, Dallabernadina, Pietro, Boos, Irene, Andersen, Mathias C. F., Kotake, Toshihisa, Knox, J. Paul, Hahn, Michael G., Clausen, Mads H., Pfrengle, Fabian
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - metabolism
BIOCHEMISTRY AND METABOLISM
Brachypodium - metabolism
Cell Wall - metabolism
Epitope Mapping
Glycoside Hydrolases - metabolism
Microarray Analysis - methods
Polysaccharides - metabolism
Staining and Labeling
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Summary:In the last three decades, more than 200 monoclonal antibodies have been raised against most classes of plant cell wall polysaccharides by different laboratories worldwide. These antibodies are widely used to identify differences in plant cell wall components in mutants, organ and tissue types, and developmental stages. Despite their importance and broad use, the precise binding epitope has been determined for only a few of these antibodies. Here, we use a plant glycan microarray equipped with 88 synthetic oligosaccharides to comprehensively map the epitopes of plant cell wall glycan-directed antibodies. Our results reveal the binding epitopes for 78 arabinogalactan-, rhamnogalacturonan-, xylan-, and xyloglucan-directed antibodies. We demonstrate that, with knowledge of the exact epitopes recognized by individual antibodies, specific glycosyl hydrolases can be implemented into immunological cell wall analyses, providing a framework to obtain structural information on plant cell wall glycans with unprecedented molecular precision.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.17.00737