Physicochemical characterization of actomyosin–paramyosin from giant squid mantle (Dosidicus gigas)

BACKGROUND The giant squid (Dosidicus gigas) has been proposed as raw material to obtain myofibrillar protein concentrates. However, it has been observed that colloidal systems formed from squid proteins have limited stability. Therefore, the isolation and characterization of the actomyosin–paramyos...

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Bibliographic Details
Published in:Journal of the science of food and agriculture 2018-03, Vol.98 (5), p.1787-1793
Main Authors: Tolano‐Villaverde, Ivan J, Ocaño‐Higuera, Victor, Ezquerra‐Brauer, Josafat, Santos‐Sauceda, Irela, Santacruz‐Ortega, Hisila, Cárdenas‐López, José L, Rodríguez‐Olibarria, Guillermo, Márquez‐Ríos, Enrique
Format: Article
Language:English
Subjects:
Actin
Actomyosin
actomyosin–paramyosin
Amino acids
Dosidicus gigas
Giant squids
Mantle
Myosin
Proteins
sulfhydryl
Sulfhydryl groups
Zeta potential
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Summary:BACKGROUND The giant squid (Dosidicus gigas) has been proposed as raw material to obtain myofibrillar protein concentrates. However, it has been observed that colloidal systems formed from squid proteins have limited stability. Therefore, the isolation and characterization of the actomyosin–paramyosin isolated (API) complex were performed, because they are the main proteins to which functionality has been attributed. RESULTS Densitogram analysis revealed 45% of actin, 38% of myosin and 17% of paramyosin. The amino acid profile indicates a higher proportion of acidic amino acids, which gives a higher negative charge; this was supported by the zeta potential. Total sulfhydryl (TSH) content was lower compared with proteins of other aquatic species. CONCLUSION The higher percentage of actin in relation to myosin, the presence of paramyosin, as well as the low content of sulfhydryl groups, could comprise the main causes of the low technological functional property of proteins from D. gigas mantle. © 2017 Society of Chemical Industry
ISSN:0022-5142
1097-0010
DOI:10.1002/jsfa.8653