Performance of the multitarget Mikrogen Chlamydia trachomatis IgG ELISA in the prediction of tubal factor infertility (TFI) in subfertile women: comparison with the Medac MOMP IgG ELISA plus

Abstract There is a need for more accurate Chlamydia trachomatis (CT) IgG antibody tests for tubal factor infertility (TFI) diagnostics. We evaluated the predictive value for TFI of Medac ELISA plus (MOMP) and multitarget Mikrogen ELISA (MOMP-CPAF-TARP). Based on Medac ELISA plus results, 183 subfer...

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Bibliographic Details
Published in:Pathogens and disease 2017-10, Vol.75 (7)
Main Authors: van Ess, Eleanne F., Ouburg, Sander, Spaargaren, Joke, Land, Jolande A., Morré, Servaas A.
Format: Article
Language:English
Subjects:
Adult
Antibodies, Bacterial - blood
Case-Control Studies
Chlamydia
Chlamydia Infections - complications
Chlamydia Infections - diagnosis
Chlamydia Infections - microbiology
Chlamydia Infections - pathology
Chlamydia trachomatis
Chlamydia trachomatis - pathogenicity
Chlamydia trachomatis - physiology
Computed tomography
Enzyme-Linked Immunosorbent Assay
Fallopian Tubes - microbiology
Fallopian Tubes - pathology
Female
Fertility
Humans
Hysterosalpingography
IgG antibody
Immunoglobulin G
Immunoglobulin G - blood
Infertility
Infertility, Female - complications
Infertility, Female - diagnosis
Infertility, Female - microbiology
Infertility, Female - pathology
Laparoscopy
Major outer membrane protein
Occlusion
Patients
Predictions
Sensitivity and Specificity
Sexually transmitted diseases
Tissue Adhesions - complications
Tissue Adhesions - diagnosis
Tissue Adhesions - microbiology
Tissue Adhesions - pathology
Womens health
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Summary:Abstract There is a need for more accurate Chlamydia trachomatis (CT) IgG antibody tests for tubal factor infertility (TFI) diagnostics. We evaluated the predictive value for TFI of Medac ELISA plus (MOMP) and multitarget Mikrogen ELISA (MOMP-CPAF-TARP). Based on Medac ELISA plus results, 183 subfertile women underwent either hysterosalpingography or laparoscopy to diagnose TFI. TFI was defined as extensive adhesions and/or distal occlusion of at least one tube. Women not fulfilling the definition of TFI served as controls. Serum was subsequently tested with Mikrogen ELISA and results were compared. 48 patients had TFI, 135 were controls. Mikrogen ELISA tested 125 patients positive/borderline of which 32% had TFI. Medac ELISA plus tested 77 patients positive/borderline of which 29.9% had TFI. Mikrogen tested 40 out of 48 TFI patients positive/borderline, Medac 23 out of 48. Kappa value was 0.34. PPV of Mikrogen ELISA and Medac ELISA plus were respectively 32% (95% CI 26%–39%) and 30% (95% CI 24%–37%), and NPV 86% (95% CI 81%–91%) and 76% (95% CI 70%–82%). Both tests were comparable in the prediction of TFI. However, Mikrogen ELISA had a higher NPV and might be more reliable in identifying patients without TFI. Kappa-value showed limited concordance between both tests. This study compares the predictive value for Chlamydia trachomatis induced tubal factor infertility of a mono-target CT IgG ELISA to a multitarget CT IgG ELISA in order to improve the fertility work-up.
ISSN:2049-632X
2049-632X
DOI:10.1093/femspd/ftx067