Galectin‐1 from conditioned medium of three‐dimensional culture of adipose‐derived stem cells accelerates migration and proliferation of human keratinocytes and fibroblasts

Keratinocytes and fibroblasts cells play important roles in the skin‐wound healing process and are the cell types activated by trauma. Activated cells participate in epithelialization, granulation, scar tissue formation, wound remodeling, and angiogenesis via a series of cellular activities includin...

Full description

Saved in:
Bibliographic Details
Published in:Wound repair and regeneration 2018-12, Vol.26 (S1), p.S9-S18
Main Authors: Kim, Min Ho, Wu, Wen Hao, Choi, Jee Hyun, Kim, Jihyun, Jun, Jin Hyun, Ko, Yong, Lee, Jong Hun
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Cell Migration Assays
Cell Proliferation
Cells, Cultured
Culture Media, Conditioned - pharmacology
Fibroblasts
Galectin 1 - metabolism
Humans
In Vitro Techniques
Keratinocytes - metabolism
Proteomics
Re-Epithelialization - physiology
Skin - injuries
Skin - pathology
Stem Cells
Wound Healing - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Keratinocytes and fibroblasts cells play important roles in the skin‐wound healing process and are the cell types activated by trauma. Activated cells participate in epithelialization, granulation, scar tissue formation, wound remodeling, and angiogenesis via a series of cellular activities including migration and proliferation. Previous studies reported that the conditioned medium (CM) of adipose‐derived stem cells (ADSCs) stimulated the migration and proliferation of cell types involved in the skin wound healing process; however, these studies only show ADSC‐CM effects that were obtained using 2‐dimensional (2D) culture. Recently, 3‐dimensional (3D) culture has been considered as a more physiologically appropriate system than 2D culture for ADSC cultures; therefore, ADSC‐CM was collected from 3D culture (ADSC‐CM‐3D) and compared with ADSC‐CM from 2D culture (ADSC‐CM‐2D) to investigate the effects on the migration and proliferation of human keratinocytes (HaCaTs) and fibroblasts. The migrations of the HaCaT cells and fibroblasts were significantly higher with ADSC‐CM‐3D compared with the 2D culture; similarly, the proliferation of HaCaT cells was also highly stimulated by ADSC‐CM‐3D. Proteomic analyses of the ADSC‐CM revealed that collagens and actins were highly expressed in the 3D‐culture system. Chitinase 3‐like 1 (CHI3L1), tissue inhibitor of metalloproteinases (TIMP), and galectin‐1 were specifically expressed only in ADSC‐CM‐3D. Especially, through antibody neutralization, galectin‐1 in ADSC‐CM‐3D was found to be an important factor for the migration of human keratinocytes. Therefore, these results suggest that ADSC‐CM‐3D was more effective in the wound healing than ADSC‐CM‐2D, and galectin‐1 in ADSC‐CM‐3D was could be a promising option for skin‐wound healing. Furthermore, the differential expressions of several ADSC‐CM proteins between the 2D‐ and 3D‐culture systems may be used as basic information for the development of efficient wound‐healing strategies.
ISSN:1067-1927
1524-475X
DOI:10.1111/wrr.12579