Possibility of Aggravation of Tissue Sclerosis after Injection of Multipotent Mesenchymal Stromal Cells Near the Forming Cicatrix in the Experiment

The peculiarities of tissue sclerosis after injection of autologous bone marrow multipotent mesenchymal stromal cells transfected with GFP gene and stained with Vybrant CM-Dil cell membrane dye were studied by light microscopy with luminescence. The surgical intervention consisting in ligation of th...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2017-08, Vol.163 (4), p.554-560
Main Authors: Maiborodin, I. V., Morozov, V. V., Anikeev, A. A., Figurenko, N. F., Maslov, R. V., Matveeva, V. A., Chastikina, G. A., Maiborodina, V. I.
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Bone Marrow Cells - cytology
Bone Marrow Cells - physiology
Cell Biology
Cell Differentiation - physiology
Cicatrix - metabolism
Collagen
Inflammation
Internal Medicine
Laboratory Medicine
Male
Mesenchymal Stem Cell Transplantation
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - physiology
Pathology
Rats
Sclerosis - therapy
Stem cells
Stromal Cells - cytology
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
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Summary:The peculiarities of tissue sclerosis after injection of autologous bone marrow multipotent mesenchymal stromal cells transfected with GFP gene and stained with Vybrant CM-Dil cell membrane dye were studied by light microscopy with luminescence. The surgical intervention consisting in ligation of the great vein was followed by tissue sclerotic transformation caused by direct damage and chronic inflammation caused by the presence of slowly resorbed ligature. Injection of stromal cells after this intervention led to formation of more extensive scar. This can attest to the possibility of stromal cells differentiation into connective tissue cells, fibroblasts, and stimulation of proliferation and collagen synthesis by host fibroblasts. A decrease in the volume of dense fibrous connective tissue due to scar reorganization at latter terms cannot not excluded.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-017-3848-1