Anti-inflammatory sesquiterpenoids from the Traditional Chinese Medicine Salvia plebeia: Regulates pro-inflammatory mediators through inhibition of NF-κB and Erk1/2 signaling pathways in LPS-induced Raw264.7 cells

Salvia plebeia R. Brown, a traditional Chinese medicinal herb, has been used to treat inflammatory diseases such as cough, hepatitis, and diarrhea for a long history. The aim of the present study was to isolate and identify potential anti-inflammatory agents from the herb of S. plebeia, which may ha...

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Bibliographic Details
Published in:Journal of ethnopharmacology 2018-01, Vol.210, p.95-106
Main Authors: Zou, Yi-Hong, Zhao, Liang, Xu, You-Kai, Bao, Jing-Mei, Liu, Xin, Zhang, Jun-Sheng, Li, Wei, Ahmed, Abrar, Yin, Sheng, Tang, Gui-Hua
Format: Article
Language:English
Subjects:
Animals
Anti-inflammation
Anti-Inflammatory Agents - isolation & purification
Anti-Inflammatory Agents - pharmacology
Chromatography, High Pressure Liquid - methods
Cytokines - metabolism
Disease Models, Animal
I-kappa B Proteins - metabolism
Inflammation - drug therapy
Inflammation Mediators - metabolism
Lipopolysaccharides - administration & dosage
Macrophages - drug effects
Macrophages - metabolism
MAP Kinase Signaling System - drug effects
Medicine, Chinese Traditional
Mice
NF-kappa B - metabolism
NF-κB
Plant Components, Aerial
Plant Extracts - pharmacology
RAW 264.7 Cells
Salvia - chemistry
Salvia plebeia
Salviplenoid A
Sesquiterpenes - isolation & purification
Sesquiterpenes - pharmacology
Sesquiterpenoid
Signal Transduction - drug effects
Tumor Necrosis Factor-alpha - metabolism
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Summary:Salvia plebeia R. Brown, a traditional Chinese medicinal herb, has been used to treat inflammatory diseases such as cough, hepatitis, and diarrhea for a long history. The aim of the present study was to isolate and identify potential anti-inflammatory agents from the herb of S. plebeia, which may have contributed to its folk pharmacological use in the treatment of inflammatory diseases. The aerial parts of S. plebeia were extracted with 95% ethanol and separated by silica gel, RP-C18, Sephadex LH-20, and HPLC. The structures of the isolated compounds were elucidated by extensive spectroscopic analysis (MS, NMR, and X-ray). Anti-inflammatory activities of all compounds were evaluated by the model of LPS-induced up-regulated of NO in Raw264.7 macrophages. The expression levels of cytokine (TNF-α) and proteins (iNOS and COX-2) were assessed by ELISA kit and Western blotting analysis, respectively. Furthermore, the influences of salviplenoid A (1) on NF-κB and MAPK signaling pathways were determined by Western blotting analysis and immunofluorescence assay. Six new (1−6, salviplenoids A–F) and ten known (7–16) sesquiterpenoids were isolated from the herb of S. plebeia. The absolute configurations of compounds 1, 2, and 7 were determined by X-ray diffraction. The new eudesmane-type sesquiterpenoid, salviplenoid A (1), significantly decreased the release of NO and TNF-α and the expression of proteins iNOS and COX-2. In addition, the biochemical mechanistic study indicated that 1 regulated the NF-κB dependent transcriptional activity through inhibiting the nuclear translocation of p50/p65 dimer and decreasing the phosphorylation of IκB and Erk1/2. Among all sesquiterpenoids isolated from S. plebeian, the new salviplenoid A (1) exhibited the most potent anti-inflammatory activity in LPS-induced Raw264.7 cells via inhibition of NF-κB and Erk1/2 signaling pathways. [Display omitted]
ISSN:0378-8741
1872-7573
DOI:10.1016/j.jep.2017.08.034