Oxidative degradation of polyaromatic hydrocarbons catalyzed by blue laccase from Pleurotus ostreatus D1 in the presence of synthetic mediators

The laccase produced by the white-rot fungus Pleurotus ostreatus D1 (BLPO) under submerged cultivation was purified to homogeneity. Its catalytic activity towards several phenolic compounds, including ABTS (2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt), syringaldazine (4-h...

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Bibliographic Details
Published in:Enzyme and microbial technology 2006-10, Vol.39 (6), p.1242-1249
Main Authors: Pozdnyakova, Natalia N., Rodakiewicz-Nowak, Janina, Turkovskaya, Olga V., Haber, Jerzy
Format: Article
Language:eng ; rus
Subjects:
Degradation
Laccase
Pleurotus ostreatus
Polycyclic aromatic hydrocarbons (PAHs)
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Summary:The laccase produced by the white-rot fungus Pleurotus ostreatus D1 (BLPO) under submerged cultivation was purified to homogeneity. Its catalytic activity towards several phenolic compounds, including ABTS (2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt), syringaldazine (4-hydroxy-3,5-dimethoxybenzaldehyde azine), 2,6-dimethoxyphenol and catechol was investigated. pH-optima and kinetic constants ( K m and V max) of these reactions were determined. The enzyme was able to degrade the following polyaromatic hydrocarbons (PAHs): anthracene (91%), phenanthrene (72%), fluorene (53.5%), pyrene (65.5%), fluoranthene (69.7%), and perylene (73%) only in the presence of a synthetic mediator. The degradation of phenanthrene in the presence of detergents varied from 49% to 72%, whereas in the absence of any detergent it reached 10%. Investigating the effect of various mediators on PAHs degradation by the BLPO showed that ABTS was a better mediator of anthracene oxidation and HBT (1-hydroxybenzotriazole) was a better mediator of fluorene oxidation. Pyrene and anthracene were degraded more rapidly in a mixture than separately. The effect of different solvents on the activity of the BLPO towards anthracene and fluorene was investigated also. Finally, it was found that in a solution of 30% (v/v) acetonitrile, BLPO could degrade about 56% of the 600 μM initially added anthracene with the formation of anthraquinone.
ISSN:0141-0229
1879-0909
DOI:10.1016/j.enzmictec.2006.03.009