Phytochelatins do not correlate with the level of Cd accumulation in Chlamydomonas spp

Chlamydomonas acidophila KT-1 and Chlamydomonas acidophila DVB238 exhibit a strong heavy metal tolerance, but C. acidophila DVB238 can accumulate a much higher amount of Cadmium (Cd) than C. acidophila KT-1. Phytochelatins (PCs) are known to play an important role in the detoxification of several to...

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Bibliographic Details
Published in:Chemosphere (Oxford) 2006-06, Vol.63 (9), p.1553-1559
Main Authors: Nishikawa, Kahoko, Onodera, Ai, Tominaga, Noriko
Format: Article
Language:English
Subjects:
Aminoacyltransferases - metabolism
Animal, plant and microbial ecology
Animals
Applied ecology
bioaccumulation
Biological and medical sciences
biosynthesis
cadmium
Cadmium - metabolism
Cadmium - pharmacology
Cd accumulation
Chlamydomonas
Chlamydomonas - drug effects
Chlamydomonas - metabolism
Chlamydomonas - ultrastructure
Chlamydomonas acidophila
Chlamydomonas reinhardtii
chloroplasts
Cysteine - metabolism
cytochemistry
cytosol
Dose-Response Relationship, Drug
Ecotoxicology, biological effects of pollution
Fundamental and applied biological sciences. Psychology
General aspects
Glutamate-Cysteine Ligase - metabolism
Glutathione
Glutathione - metabolism
Heavy metal tolerance
Inactivation, Metabolic
metabolic detoxification
Phytochelatin
Phytochelatins
starch granules
vacuoles
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Summary:Chlamydomonas acidophila KT-1 and Chlamydomonas acidophila DVB238 exhibit a strong heavy metal tolerance, but C. acidophila DVB238 can accumulate a much higher amount of Cadmium (Cd) than C. acidophila KT-1. Phytochelatins (PCs) are known to play an important role in the detoxification of several toxic heavy metals, but the relationship between PCs and Cd accumulation is not clear. PC metabolism and Cd accumulation were investigated by using three Chlamydomonas strains including Chlamydomonas reinhardtii C-9 as a standard alga. The results showed that the PC content did not correlate closely with the level of Cd accumulation, maintenance of a high GSH level seeming to be more important for Cd accumulation. The ultrastructure of C. acidophila KT-1 was extremely disrupted by a great increase in starch granules, which resulted in a moribund state, but hyper-accumulator C. acidophila DVB238 did not exhibit an increase in starch granules in its cells, in spite of Cd accumulation in its chloroplasts, cytosol and vacuoles. These results indicated that C. acidophila DVB238 probably has a developed detoxification system preventing such as destruction of the cells due to Cd toxicity.
ISSN:0045-6535
1879-1298
DOI:10.1016/j.chemosphere.2005.09.056