Basidiomycete‐specific PsCaMKL1 encoding a CaMK‐like protein kinase is required for full virulence of Puccinia striiformis f. sp. tritici

Summary Calcium/calmodulin‐dependent kinases (CaMKs) are Ser/Thr protein kinases (PKs) that respond to changes in cytosolic free Ca2+ and play diverse roles in eukaryotes. In fungi, CAMKs are generally classified into four families CAMK1, CAMKL, RAD53 and CAMK‐Unique. Among these, CAMKL constitutes...

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Published in:Environmental microbiology 2017-10, Vol.19 (10), p.4177-4189
Main Authors: Jiao, Min, Yu, Dan, Tan, Chenglong, Guo, Jia, Lan, Dingyun, Han, Ershang, Qi, Tuo, Voegele, Ralf Thomas, Kang, Zhensheng, Guo, Jun
Format: Article
Language:English
Subjects:
Agricultural production
Amino Acid Sequence - genetics
Base Sequence
Basidiomycota - genetics
Basidiomycota - pathogenicity
Biomedical materials
Ca2+/calmodulin-dependent protein kinase
Calcium
Calcium-binding protein
Calcium-Calmodulin-Dependent Protein Kinases - genetics
Calmodulin
Environmental stress
Eukaryotes
Fungi
Gene expression
Gene Knockdown Techniques
Gene silencing
Hypersensitive response
Kinases
Pathogenesis
Pathogenicity
Pathogens
Pk gene
Plant Diseases - microbiology
Polymorphism
Puccinia
Reactive oxygen species
Reactive Oxygen Species - metabolism
Rust fungi
Sequence Analysis, DNA
Surgical implants
Triticum - microbiology
Virulence
Virulence Factors - genetics
Wheat
Yeast
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Summary:Summary Calcium/calmodulin‐dependent kinases (CaMKs) are Ser/Thr protein kinases (PKs) that respond to changes in cytosolic free Ca2+ and play diverse roles in eukaryotes. In fungi, CAMKs are generally classified into four families CAMK1, CAMKL, RAD53 and CAMK‐Unique. Among these, CAMKL constitutes the largest family. In some fungal plant pathogens, members of the CaMKL family have been shown to be responsible for pathogenesis. However, little is known about their role(s) in rust fungi. In this study, we functionally characterized a novel PK gene, PsCaMKL1, from Puccinia striiformis f. sp. tritici (Pst). PsCaMKL1 belongs to a group of PKs that is evolutionarily specific to basidiomyceteous fungi. PsCaMKL1 shows little intra‐species polymorphism between Pst isolates. PsCaMKL1 transcripts are highly elevated at early infection stages, whereas gene expression is downregulated in barely germinated urediospores under KN93 treatment. Overexpression of PsCaMKL1 in fission yeast increased resistance to environmental stresses. Knock down of PsCaMKL1 using host‐induced gene silencing (HIGS) reduced the virulence of Pst accompanied by reactive oxygen species (ROS) accumulation and a hypersensitive response. These results suggest that PsCaMKL1 is a novel pathogenicity factor that exerts it virulence function by regulating ROS production in wheat.
ISSN:1462-2912
1462-2920
DOI:10.1111/1462-2920.13881