Differential behavioral effects of ethanol pre-exposure in male and female zebrafish (Danio rerio)

[Display omitted] •Ethanol pre-exposure of juvenile zebrafish affected distinct behavioral responses of adult male and female zebrafish.•Pre-exposure altered ethanol conditioning dose- and sex-dependent percent time on the non-preferred tank side (PTN).•The PTN response of individual zebrafish was p...

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Bibliographic Details
Published in:Behavioural brain research 2017-09, Vol.335, p.174-184
Main Authors: Clayman, Carly L., Malloy, Elizabeth J., Kearns, David N., Connaughton, Victoria P.
Format: Article
Language:English
Subjects:
Alcohol
Conditioned place preference
Juvenile
Novel tank
Shoaling
Zebrafish
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Summary:[Display omitted] •Ethanol pre-exposure of juvenile zebrafish affected distinct behavioral responses of adult male and female zebrafish.•Pre-exposure altered ethanol conditioning dose- and sex-dependent percent time on the non-preferred tank side (PTN).•The PTN response of individual zebrafish was predictive of absolute angular velocity during ethanol conditioning.•Ethanol conditioning-induced locomotor behavior was influenced by ethanol conditioning dose, sex, and ethanol pre-exposure.•Female zebrafish displayed increased anxiety-like behavior in a novel tank and shoaling regardless of ethanol pre-exposure. Alcohol exposure in adolescence is a contributing factor toward reward-seeking behavior in adulthood. This reward-seeking behavior is assessed in animal models using the conditioned place preference (CPP) paradigm. In this study, ethanol-induced change in time spent by zebrafish on the initially non-preferred tank side was studied by conditioning adult zebrafish to ethanol dissolved in water (0.00% 1.00%; 1.25%; 1.50%; 1.60%; 1.75% vol/vol) paired with an initially non-preferred environment. Following a single conditioning cycle, fish swam unrestricted in the CPP chamber to assess changes in preference. Daily 20-min pre-exposure to ethanol for 1 week during the juvenile stage starting at either 20days post fertilization (dpf) or 40 dpf altered percent time spent on the ethanol-paired side in adulthood in a dose-dependent and sex-dependent manner. The results suggest that male and female zebrafish are an effective model in which to investigate behavioral correlates of ethanol-induced changes in neural circuits implicated in reward and anxiety.
ISSN:0166-4328
1872-7549
DOI:10.1016/j.bbr.2017.08.007