Adoption of the Q Transcriptional System for Regulating Gene Expression in Stem Cells

The field of mammalian synthetic biology seeks to engineer enabling technologies to create novel approaches for programming cells to probe, perturb, and regulate gene expression with unprecedented precision. To accomplish this, new genetic parts continue to be identified that can be used to build no...

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Bibliographic Details
Published in:ACS synthetic biology 2017-11, Vol.6 (11), p.2014-2020
Main Authors: Fitzgerald, Michael, Gibbs, Chelsea, Shimpi, Adrian A, Deans, Tara L
Format: Article
Language:English
Subjects:
Animals
Cell Engineering - methods
CHO Cells
Cricetulus
Gene Expression Regulation
HEK293 Cells
Humans
Mice
Mouse Embryonic Stem Cells - metabolism
Transcription, Genetic
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Summary:The field of mammalian synthetic biology seeks to engineer enabling technologies to create novel approaches for programming cells to probe, perturb, and regulate gene expression with unprecedented precision. To accomplish this, new genetic parts continue to be identified that can be used to build novel genetic circuits to re-engineer cells to perform specific functions. Here, we establish a new transcription-based genetic circuit that combines genes from the quinic acid sensing metabolism of Neorospora crassa and the bacterial Lac repressor system to create a new orthogonal genetic tool to be used in mammalian cells. This work establishes a novel genetic tool, called LacQ, that functions to regulate gene expression in Chinese hamster ovarian (CHO) cells, human embryonic kidney 293 (HEK293) cells, and in mouse embryonic stem (ES) cells.
ISSN:2161-5063
2161-5063
DOI:10.1021/acssynbio.7b00149