Bioluminescent bioreporter integrated circuits: potentially small, rugged and inexpensive whole‐cell biosensors for remote environmental monitoring

A bioluminescent bioreporter integrated circuit (BBIC) is a novel whole-cell biosensor that combines the environmental monitoring capabilities of genetically engineered bioluminescent micro-organisms (bioreporters) with optical application-specific integrated circuits. A BBIC device consists of bior...

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Bibliographic Details
Published in:Journal of applied microbiology 2004-01, Vol.96 (1), p.33-46
Main Authors: Nivens, D.E., McKnight, T.E., Moser, S.A., Osbourn, S.J., Simpson, M.L., Sayler, G.S.
Format: Article
Language:English
Subjects:
Bacteria
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Biotechnology
Electronics
Environmental Monitoring - instrumentation
Environmental Monitoring - methods
Fundamental and applied biological sciences. Psychology
Genes, Reporter
Luminescent Measurements
Methods. Procedures. Technologies
Pseudomonas fluorescens
Various methods and equipments
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Summary:A bioluminescent bioreporter integrated circuit (BBIC) is a novel whole-cell biosensor that combines the environmental monitoring capabilities of genetically engineered bioluminescent micro-organisms (bioreporters) with optical application-specific integrated circuits. A BBIC device consists of bioreporters sustained within a micro-environment, the integrated circuit microluminometer, and a light-tight enclosure. The bioreporter typically contains the luxCDABE reporter genes encoding the enzymes required for bioluminescence. In the presence of a targeted analyte, a gene (transcriptional) regulatory system induces the expression of the luxCDABE genes. Analytical benchmark data for exposure of the bioreporter Pseudomonas fluorescens 5RL to salicylate was determined using a flow-through test system. The detection limit (after a 45 min exposure) was ca 50 mu 1 super(-1) and response times decreased from ca 45 to 20 min as the concentration increased from 50 mu 1 super(-1) to 1 mg 1 super(-1). These results are currently being used to scrutinize enclosures and micro-environment configurations to devlop a simple and inexpensive means of creating a 'laboratory-on-a-chip' and could be used in a network to protect valuable human and environmental resources. This article reviews the present state of luxCDABE-based bioreporter research, demonstrates the integration of the bioreporters with complementary metal oxide semiconductor photodiode-integrated circuits, and discusses future challenges for real-time in situ BBIC environmental monitoring.
ISSN:1364-5072
1365-2672
DOI:10.1046/j.1365-2672.2003.02114.x