γ-Radiation Decontamination of Alfalfa Seeds Naturally Contaminated with Salmonella Mbandaka

ABSTRACT Samples of alfalfa seeds inoculated with Salmonella Mbandaka isolated from a naturally contaminated lot were γ‐irradiated to 1 of 8 doses between 0 and 2.8 kGy, producing a maximum inactivation of 3.3 logs CFU/g. Analysis of the data indicates that the γ‐radiation D value is 0.81 ± 0.02 kGy...

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Bibliographic Details
Published in:Journal of food science 2003-06, Vol.68 (5), p.1777-1781
Main Authors: Thayer, D.W., Boyd, G., Fett, W.F.
Format: Article
Language:English
Subjects:
alfalfa
Biological and medical sciences
Food industries
Food irradiation
Food science
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Mbandaka
Pathogens
Salmonella
seed
Seeds
γ-radiation
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Summary:ABSTRACT Samples of alfalfa seeds inoculated with Salmonella Mbandaka isolated from a naturally contaminated lot were γ‐irradiated to 1 of 8 doses between 0 and 2.8 kGy, producing a maximum inactivation of 3.3 logs CFU/g. Analysis of the data indicates that the γ‐radiation D value is 0.81 ± 0.02 kGy. An absorbed dose of 4 kGy, but not 3 kGy, eliminated viable S. Mbandaka from naturally contaminated seeds. Results of experiments with different percentages of inoculated seeds indicate that the dose required to inactivate a contaminating pathogen on alfalfa seeds is dependent upon the maximum contamination per seed, not the mean contamination of the lot.
ISSN:0022-1147
1750-3841
DOI:10.1111/j.1365-2621.2003.tb12328.x