Association of incretin receptors genetic polymorphisms with type 2 diabetes mellitus in Egyptian patients

Background Incretins have opened a new era in type 2 diabetes mellitus (T2DM) pathogenesis. The present study aimed to assess whether there is an association between GIPR rs2302382, GIPR rs1800437 and GLP‐1R rs367543060 polymorphisms with T2DM or not and also to determine the effect of these polymor...

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Bibliographic Details
Published in:The journal of gene medicine 2017-09, Vol.19 (9-10), p.n/a
Main Authors: Shalaby, Sally M., Zidan, Haidy E., Shokry, Amira, Saeed, Jehan, El‐Sokkary, Rehab H.
Format: Article
Language:English
Subjects:
Adult
Alleles
Biomarkers
Case-Control Studies
Diabetes
Diabetes mellitus
Diabetes Mellitus, Type 2 - blood
Diabetes Mellitus, Type 2 - epidemiology
Diabetes Mellitus, Type 2 - genetics
Egypt - epidemiology
Enzyme-linked immunosorbent assay
Female
Gene Frequency
Gene therapy
Genetic Predisposition to Disease
Genotype
GIP
GLP‐1
Glucagon
Glucagon-like peptide 1
Glucagon-Like Peptide Receptors - blood
Glucagon-Like Peptide Receptors - genetics
Haplotypes
Humans
incretins
Male
Middle Aged
Odds Ratio
Polymerase chain reaction
polymorphism
Polymorphism, Genetic
Polymorphism, Single Nucleotide
Receptors, Gastrointestinal Hormone - genetics
Restriction fragment length polymorphism
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Summary:Background Incretins have opened a new era in type 2 diabetes mellitus (T2DM) pathogenesis. The present study aimed to assess whether there is an association between GIPR rs2302382, GIPR rs1800437 and GLP‐1R rs367543060 polymorphisms with T2DM or not and also to determine the effect of these polymorphisms on gastric inhibitory polypeptide (GIP) and glucagon‐like peptide‐1 (GLP‐1) levels. Methods One hundred and fifty T2DM patients and 150 healthy controls were included in the study. Polymorphisms of GIPR rs1800437, GIPR rs2302382 and GLP‐1R rs367543060 were genotyped using restriction fragment length polymorphism (RFLP)‐polymerase chain reaction (PCR), multiplex allele‐specific PCR and RFLP‐PCR respectively. GIP and GLP levels were measured by an enzyme‐linked immunosorbent assay. Results We found a significant association of both the homozygous AA and the minor allele A of GIPR rs2302382 with T2DM. The frequency of haplotype C(rs2302382) G(rs1800437) was significantly higher in controls than in diabetics; odds ratio (95% confidence interval): 1.99 (1.44–2.75) (p 
ISSN:1099-498X
1521-2254
DOI:10.1002/jgm.2973