Drug resistance and Mycobacterium tuberculosis strain diversity in TB/HIV co-infected patients in Ho Chi Minh city, Vietnam

•TB/HIV co-infection in Vietnam was associated with high rates of TB drug resistance and likely community transmission of drug resistant strains.•The vast majority of isoniazid resistant strains in Vietnam have high-level isoniazid resistance in HIV (+) patients.•Beijing is the predominant lineage a...

Full description

Saved in:
Bibliographic Details
Published in:Journal of global antimicrobial resistance. 2017-09, Vol.10, p.154-160
Main Authors: Mai, Trinh Quynh, Van Anh, Nguyen Thi, Hien, Nguyen Tran, Lan, Nguyen Huu, Giang, Do Chau, Hang, Pham Thi Thu, Lan, Nguyen Thi Ngoc, Marais, Ben J., Sintchenko, Vitali
Format: Article
Language:English
Subjects:
Adolescent
Adult
Antitubercular Agents - pharmacology
Bacterial Proteins - genetics
Catalase - genetics
Coinfection
DNA-Directed RNA Polymerases - genetics
Drug resistance
Drug Resistance, Multiple, Bacterial - genetics
Epidemiology
Female
Genes, Bacterial - genetics
HIV Infections - complications
HIV Infections - epidemiology
Humans
Male
Microbial Sensitivity Tests
Middle Aged
Mutation
Mycobacterium tuberculosis
Mycobacterium tuberculosis - drug effects
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - isolation & purification
Mycobacterium tuberculosis - pathogenicity
Oxidoreductases - genetics
Pentosyltransferases - genetics
Phylogeny
TB/HIV co-infection
Tuberculosis
Tuberculosis, Multidrug-Resistant - complications
Tuberculosis, Multidrug-Resistant - epidemiology
Tuberculosis, Multidrug-Resistant - microbiology
Vietnam
Vietnam - epidemiology
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•TB/HIV co-infection in Vietnam was associated with high rates of TB drug resistance and likely community transmission of drug resistant strains.•The vast majority of isoniazid resistant strains in Vietnam have high-level isoniazid resistance in HIV (+) patients.•Beijing is the predominant lineage among TB/HIV co-infected patients; but EAI-5 is specifically overrepresented compared to HIV (−) patients.•The variety of drug resistance profiles and phylogenetic diversity of strains do not suggest high rates of nosocomial transmission. Mycobacterium tuberculosis strain diversity and drug resistance among people living with human immunodeficiency virus (HIV) in Vietnam have not been described previously. We examined M. tuberculosis isolates from TB/HIV co-infected patients in Ho Chi Minh City, Vietnam. Drug susceptibility testing (DST), spoligotyping and 24-locus Mycobacterial Interspersed Repetitive Unit (MIRU-24 typing) were performed, and the rpoB, katG, inhA and inhA promoter, rpsL, rrs and embB genes were sequenced in all drug resistant isolates identified. In total, 84/200 (42.0%) strains demonstrated “any drug resistance”; 17 (8.5%) were multi-drug resistant (MDR). Streptomycin resistance was present in 80 (40.0%) isolates; 95.2% (80/84) with “any drug resistance” and 100% with MDR. No rifampicin monoresistance was detected. Of the rifampicin resistant strains 16/18 (88.9%) had mutations in the 81-bp Rifampicin Resistance Defining Region (RRDR) of the rpoB gene. Isoniazid resistance was mostly associated with Ser315Thr mutations in the katG gene (15/17; 88.2%). Beijing (49.0%) and East African Indian (EAI) lineage strains (35.0%; 56/70 EAI-5) were most common. TB/HIV co-infection in Vietnam was associated with high rates of TB drug resistance, although we were unable to differentiate new from retreatment cases.
ISSN:2213-7165
2213-7173
DOI:10.1016/j.jgar.2017.07.003