Inherent inferior quality of follicular fluid in repeat breeder heifers as evidenced by low rates of in vitro production of bovine embryos

The aims of the present study were to determine the effect of follicular fluid obtained from the ovulatory follicle of repeat breeder heifers on in vitro oocyte maturation (Experiment 1), fertilization (Experiment 2) and production of bovine embryos (Experiment 3). Holstein virgin heifers (VH, n = 5...

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Bibliographic Details
Published in:Theriogenology 2017-10, Vol.102, p.29-34
Main Authors: Kafi, M., Azari, M., Chashnigir, O., Gharibzadeh, S., Aghabozorgi, Z., Asaadi, A., Divar, M.R.
Format: Article
Language:English
Subjects:
Animals
Bovine
Cattle - embryology
Embryo
Embryo Culture Techniques - veterinary
Female
Fertilization in Vitro - veterinary
Follicular fluid
Follicular Fluid - physiology
In vitro
Lipopolysaccharides
Ovarian Follicle - physiology
Ovulatory follicle
Progesterone - chemistry
Progesterone - physiology
Repeat breeder heifers
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Summary:The aims of the present study were to determine the effect of follicular fluid obtained from the ovulatory follicle of repeat breeder heifers on in vitro oocyte maturation (Experiment 1), fertilization (Experiment 2) and production of bovine embryos (Experiment 3). Holstein virgin heifers (VH, n = 5) with normal fertility or repeat breeder syndrome (RBH, n = 5) were used in the present study. Follicular fluid of VH and RBH was aspirated from ovulatory follicles and used as maturation medium. Bovine oocytes were aspirated from follicles of slaughterhouse ovaries and randomly allocated in three groups; in Group 1, oocytes cultured in TCM-199 supplemented with 10% heat-treated fetal calf serum and hormones (5 IU/mL hCG plus 0.1 IU/mL rFSH); in Group 2, oocytes cultured in TCM-199 supplemented with 10% filtered follicular fluid of VH without hormones; in Group 3, oocytes cultured in TCM-199 supplemented with 10% filtered follicular fluid of RBH without hormones. The mean (±SEM) percentage of matured oocytes was different between VH and RBH groups (72.2 ± 4.0 vs 56.4 ± 4.6%; P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2017.07.011